Document Detail

Intranucleolar localization of DNA topoisomerase IIalpha is a distinctive feature of necrotic, but not of apoptotic, Jurkat T-cells.
MedLine Citation:
PMID:  14506684     Owner:  NLM     Status:  MEDLINE    
Two distinct types of cell death have been described: apoptosis and necrosis. However, it is becoming increasingly clear that the differences between these two types are far less numerous than initially thought. Morphological analyses might provide important information to distinguish apoptotic from necrotic samples. We recently reported that in necrotic, but not apoptotic, HL-60 human myeloid leukaemia cells, the nuclear protein topoisomerase IIalpha concentrated in nucleoli. In order to ascertain whether or not this phenomenon was restricted to a peculiar cell type or could be detected also in cells of lymphoid lineage, we performed an investigation aimed at defining the localization of topoisomerase IIalpha in apoptotic and necrotic Jurkat human T lymphoblastoid cells. Immunofluorescence staining demonstrated that topoisomerase IIalpha was excluded from the condensed chromatin of apoptotic cells, whereas in necrotic cells it was localized in discrete nuclear dots. Immuno-electron microscopy analysis showed that topoisomerase IIalpha was undetectable in nucleoli of normal and apoptotic cells, whereas it was present in the nucleolus of necrotic cells irrespectively of the type of inducer used (ethanol, H(2)O(2), HgCl(2)). Taken together, our findings identify topoisomerase IIalpha as a potential morphological marker useful to discriminate between apoptotic and necrotic cells.
Elisabetta Falcieri; Sabrina Burattini; Roberta Bortul; Francesca Luchetti; Giovanna Tabellini; Pier Luigi Tazzari; Alessandra Cappellini; Lucio Cocco; Alberto M Martelli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microscopy research and technique     Volume:  62     ISSN:  1059-910X     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-24     Completed Date:  2003-12-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  192-200     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Istituto di Scienze Morfologiche, Campus Scientifico, Località Crocicchia, Università di Urbino, 61029 Urbino, Italy.
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MeSH Terms
Antigens, Neoplasm
Cell Differentiation
Cell Nucleus / metabolism,  ultrastructure
DNA Topoisomerases, Type II, Eukaryotic / metabolism*
DNA-Binding Proteins
Fluorescent Antibody Technique
Jurkat Cells / enzymology*,  pathology
Microscopy, Electron
Reg. No./Substance:
0/Antigens, Neoplasm; 0/DNA-Binding Proteins; EC 5.99.1.-/DNA Topoisomerases, Type II, Eukaryotic; EC topoisomerase II alpha

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