| Intranasal administration of adjuvant-combined recombinant influenza virus HA vaccine protects mice from the lethal H5N1 virus infection. | |
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MedLine Citation:
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PMID: 16968669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Attenuated recombinant H5N1 influenza virus was constructed to develop a safe H5N1 influenza vaccine. The immunogenicity and protective effect of the vaccine prepared from haemagglutinin-modified recombinant H5N1 influenza virus was evaluated in mice intranasally co-administered with cholera toxin B subunit containing a trace amount of holotoxin (CTB*), synthetic double-stranded RNA, poly (I:C) or chitin microparticles (CMP) as adjuvants. Intranasal administration of recombinant H5 HA split vaccine with CTB* or poly(I:C) and/or CMP elicited an immunological response with both anti-H5 HA IgA in the nasal wash and anti-H5 HA IgG antibody in the serum, and showed a protective against lethal H5N1 A/Hong Kong/483/97 (HK483) infection. We also demonstrated that intranasal co-administration of antigen with both poly (I:C) and CMP enhanced the expression of Toll-like receptor (TLR) 3, TLR7 in the spleen. These results indicate that poly (I:C) and CMP are highly effective as mucosal adjuvants for use with the nasal H5N1 vaccine. |
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Authors:
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Yasuko Asahi-Ozaki; Shigeyuki Itamura; Takeshi Ichinohe; Peter Strong; Shin-Ichi Tamura; Hidehiro Takahashi; Hirofumi Sawa; Masami Moriyama; Masato Tashiro; Tetsutaro Sata; Takeshi Kurata; Hideki Hasegawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-08-28 |
Journal Detail:
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Title: Microbes and infection / Institut Pasteur Volume: 8 ISSN: 1286-4579 ISO Abbreviation: Microbes Infect. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-11-10 Completed Date: 2007-02-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883508 Medline TA: Microbes Infect Country: France |
Other Details:
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Languages: eng Pagination: 2706-14 Citation Subset: IM |
Affiliation:
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Department of Pathology, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adjuvants, Immunologic Administration, Intranasal Animals Antibodies, Viral / analysis*, blood Chitin / immunology Cholera Toxin / immunology Disease Models, Animal Female Hemagglutinin Glycoproteins, Influenza Virus / genetics, immunology* Immunity, Mucosal Immunoglobulin A / analysis Immunoglobulin G / blood Influenza A Virus, H5N1 Subtype / immunology* Influenza Vaccines / administration & dosage, immunology* Lymphoid Tissue / immunology Mice Mice, Inbred BALB C Microspheres Nasal Mucosa / immunology Orthomyxoviridae Infections / prevention & control*, virology Poly I-C / immunology RNA, Double-Stranded / immunology Spleen / immunology Toll-Like Receptors / biosynthesis Vaccines, Subunit / genetics, immunology Vaccines, Synthetic / genetics, immunology |
| Chemical | |
Reg. No./Substance:
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0/Adjuvants, Immunologic; 0/Antibodies, Viral; 0/Hemagglutinin Glycoproteins, Influenza Virus; 0/Immunoglobulin A; 0/Immunoglobulin G; 0/Influenza Vaccines; 0/RNA, Double-Stranded; 0/Toll-Like Receptors; 0/Vaccines, Subunit; 0/Vaccines, Synthetic; 0/hemagglutinin, human influenza A virus; 1398-61-4/Chitin; 24939-03-5/Poly I-C; 9012-63-9/Cholera Toxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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