Document Detail


Intramyocardial sustained delivery of basic fibroblast growth factor improves angiogenesis and ventricular function in a rat infarct model.
MedLine Citation:
PMID:  12756606     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently we have demonstrated that the release of basic fibroblast growth factor (bFGF) from a biodegradable gelatin hydrogel carrier depends on the degradation of hydrogel in vivo. The purpose of our study was to assess whether bFGF-incorporating gelatin hydrogels induce myocardial angiogenesis and improve left ventricular function in the infarcted myocardium of rats. Studies were conducted in 22 Lewis rats after a 4-week ligation of the proximal left anterior descending coronary artery. The rats were randomized into the following two groups: the control group (n = 11) had an intramyocardial injection of saline alone, and the FGF group (n = 11) had gelatin hydrogel microspheres containing 100 microg of bFGF injected into the border zone of the infarct area after the repeat left thoracotomy. For visualization of the regional myocardial blood flow in the rat heart, (201)Tl images were taken just before and 4 weeks after the treatment using a 4-head single photon emission computed tomography scanner with pinhole collimators. Left ventricular function was also assessed with echocardiography and a micromanometer-tipped catheter. Finally, the extent of myocardial angiogenesis was evaluated quantitatively in the postmortem analysis. The (201)Tl defect score in the control group remained unchanged before and after the treatment, whereas it decreased significantly in the FGF group. Both regional and global left ventricular function was significantly better in the FGF group compared with the control group. The vascular density in the border zone of the infarct in the FGF group was significantly higher than that in the control group. In conclusion, intramyocardial injection of bFGF-impregnated gelatin hydrogels induces functionally significant angiogenesis and improves left ventricular systolic and diastolic function in the infarcted myocardium of rats.
Authors:
Atsushi Iwakura; Masatoshi Fujita; Kazuaki Kataoka; Keiichi Tambara; Yutaka Sakakibara; Masashi Komeda; Yasuhiko Tabata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Heart and vessels     Volume:  18     ISSN:  0910-8327     ISO Abbreviation:  Heart Vessels     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-20     Completed Date:  2003-10-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8511258     Medline TA:  Heart Vessels     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  93-9     Citation Subset:  IM    
Affiliation:
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Collateral Circulation / drug effects*
Fibroblast Growth Factor 2 / administration & dosage*,  pharmacology*,  therapeutic use
Gelatin
Hydrogel
Injections / methods
Microspheres
Myocardial Infarction / drug therapy*,  physiopathology*
Randomized Controlled Trials as Topic
Rats
Rats, Inbred Lew
Tomography, Emission-Computed, Single-Photon
Ventricular Function* / drug effects
Chemical
Reg. No./Substance:
103107-01-3/Fibroblast Growth Factor 2; 25852-47-5/Hydrogel; 9000-70-8/Gelatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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