Document Detail


Intramyocardial oxygen transport by quantitative diffuse reflectance spectroscopy in calves.
MedLine Citation:
PMID:  20459283     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intramyocardial oxygen transport was assessed during open-chest surgery in calves by diffuse reflectance spectroscopy using a small intramuscular fiber-optic probe. The sum of hemo- and myoglobin tissue fraction and oxygen saturation, the tissue fraction and oxidation of cytochrome aa3, and the tissue fraction of methemoglobin were estimated using a calibrated empirical light transport model. Increasing the oxygen content in the inhaled gas, 21%-50%-100%, in five calves (group A) gave an increasing oxygen saturation of 19+/-4%, 24+/-5%, and 28+/-8% (p<0.001, ANOVA repeated measures design) and mean tissue fractions of 1.6% (cytochrome aa3) and 1.1% (hemo- and myoglobin). Cardiac arrest in two calves gave an oxygen saturation lower than 5%. In two calves (group B), a left ventricular assistive device (LVAD pump) was implanted. Oxygen saturation in group B animals increased with LVAD pump speed (p<0.001, ANOVA) and with oxygen content in inhaled gas (p<0.001, ANOVA). The cytochrome aa3 oxidation level was above 96% in both group A and group B calves, including the two cases involving cardiac arrest. In conclusion, the estimated tissue fractions and oxygenation/oxidation levels of the myocardial chromophores during respiratory and hemodynamic provocations were in agreement with previously presented results, demonstrating the potential of the method.
Authors:
Tobias Lindbergh; Marcus Larsson; Zoltán Szabó; Henrik Casimir-Ahn; Tomas Strömberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  15     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-12     Completed Date:  2010-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  027009     Citation Subset:  IM    
Affiliation:
Linkoping University, Department of Biomedical Engineering, Division of Biomedical Instrumentation, Linkoping, 58185 Sweden. tobi@imt.liu.se
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport, Active / physiology
Cattle
Myocardium / metabolism*
Oximetry / methods*
Oxygen / analysis*
Reproducibility of Results
Sensitivity and Specificity
Spectrum Analysis / methods*
Chemical
Reg. No./Substance:
7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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