| Intramural coronary lipid injection induces atheromatous lesions expressing proinflammatory chemokines: implications for the development of a porcine model of atherosclerosis. | |
| | |
MedLine Citation:
|
PMID: 21616727 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND: Intramural delivery of lipids into the coronaries of pigs fed high-cholesterol diet results in the formation of localized atherosclerotic-like lesions within 12 weeks. These lesions are located in positively remodeled vessels and are associated to the development of abundant adventitial vasa vasorum and mononuclear cell infiltrate. In this study, we aimed to analyze the degree of expression of various inflammatory chemokines within the developed lesions compared with control segments injected with saline. METHODS: Balloon injury was performed in 15 coronary arteries of pigs fed high-cholesterol diet for 12 weeks. Two weeks after procedure, 60 coronary segments were randomized to either intramural injections of complex lipids (n=30) or normal saline (n=30). Neovessel density in the lesions was analyzed by lectin stain. Segments were processed for RNA expression of inflammatory chemokines such as monocyte chemoattractant protein-1 and vascular endothelial growth factor. RESULTS: At 12 weeks, the percentage area of stenosis seen in histological sections was modest in both groups (lipids: 17.3±15 vs. saline: 32.4±22.8, P=.017). The lipid group showed higher vasa vasorum (VV) quantity (saline: 18.2±14.9 VV/section vs. lipids: 30.6±21.6 VV/section, P<.05) and vasa vasorum density (saline: 7.3±4.6 VV/mm(2) vs. lipids: 16.5±9 VV/mm(2), P<.001). In addition, monocyte chemoattractant protein-1 expression was higher in the lipid group (1.5±1.12) compared with saline control group (0.83±0.34, P<.01). Vascular endothelial growth factor expression was also higher in the lipid group (1.36±0.9) compared with saline group (0.87±0.33, P<.05). CONCLUSION: The intramural injection of complex lipids into the coronary arteries of pigs maintained in a high-cholesterol diet results in focal lesions located in positively remodeled vessels that have a high neovessel count and express proinflammatory chemokines. |
| | |
Authors:
|
Armando Tellez; David S Schuster; Carlos Alviar; Gabriel López-Berenstein; Angela Sanguino; Christie Ballantyne; Xiao-Yuan Dai Perrard; Daryl G Schulz; Serge Rousselle; Greg L Kaluza; Juan F Granada |
Related Documents
:
|
7353977 - Applied physiology for wheelchair design. 20063807 - Effect of malnutrition on iron homeostasis in black-necked swans (cygnus melanocoryphus). 1796087 - Determination of iron absorption by rat bioassay. evaluation of methods of dosing 59fe ... 3693637 - Effect of excess iron in milk replacer on calf performance. 21779247 - Recent perspectives on the relations between fecal mutagenicity, genotoxicity, and diet. 8277517 - Cadmium-109 metabolism in mice. iv. diet versus maternal stores as a source of cadmium ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-5-24 |
Journal Detail:
|
Title: Cardiovascular revascularization medicine : including molecular interventions Volume: - ISSN: 1878-0938 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-5-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101238551 Medline TA: Cardiovasc Revasc Med Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
|
Skirball Center for Cardiovascular Research, Cardiovascular Research Foundation, Orangeburg, NY, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Embolic capture angioplasty of lower extremity lesion following distal embolization.
Next Document: An improved approach for extraction and high-performance liquid chromatography analysis of paraquat ...