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Intramedullary fixation of diaphyseal clavicle fractures using the rockwood clavicle pin: review of 86 cases.
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PMID:  23173024     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
AIM: This study reports the safety, efficacy and functional and patient centred outcomes of the largest published series of patients treated with the Rockwood clavicle pin (intramedullary device) to date.
PATIENTS AND METHODS: A retrospective review of case notes, radiographs and follow-up by questionnaire was conducted. 86 patients were operated upon, 70 for acute fractures (group A) and 16 for non-union (group B). Results: Rate of non-union was 2 (2.9%) in group A and 0 in group B. Mean Disability of the Arm, Shoulder and Hand (DASH) scores were 5.9 for group A and 8.7 for group B. Satisfaction was rated as good or excellent in 61 (96.8%) of responders and all patients would have the procedure again. Pin prominence was the predominant complication in both groups and all patients underwent a second procedure for metalwork removal.
DISCUSSION: Rockwood clavicle pins are as effective as plates in achieving union and maintaining length, however the advantages of this less invasive technique should be weighed against the common complication of pin prominence and the inconvenience of removal of metalwork in all cases.
Authors:
William J Marlow; Peter Ralte; Syam Pk Morapudi; Rashpal Bassi; Jochen Fischer; Mohammad Waseem
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Publication Detail:
Type:  Journal Article     Date:  2012-11-16
Journal Detail:
Title:  The open orthopaedics journal     Volume:  6     ISSN:  1874-3250     ISO Abbreviation:  Open Orthop J     Publication Date:  2012  
Date Detail:
Created Date:  2012-11-22     Completed Date:  2012-11-23     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  101480491     Medline TA:  Open Orthop J     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  482-7     Citation Subset:  -    
Affiliation:
Department of Trauma & Orthopaedics, Macclesfield District General Hospital, Victoria Road, Macclesfield, SK10 3BL, UK.
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Journal Information
Journal ID (nlm-ta): Open Orthop J
Journal ID (iso-abbrev): Open Orthop J
Journal ID (publisher-id): TOORTHJ
ISSN: 1874-3250
Publisher: Bentham Open
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© Marlow et al.; Licensee Bentham Open.
open-access:
Received Day: 5 Month: 8 Year: 2012
Revision Received Day: 3 Month: 10 Year: 2012
Accepted Day: 4 Month: 10 Year: 2012
Electronic publication date: Day: 16 Month: 11 Year: 2012
collection publication date: Year: 2012
Volume: 6First Page: 482 Last Page: 487
PubMed Id: 23173024
ID: 3502893
Publisher Id: TOORTHJ-6-482
DOI: 10.2174/1874325001206010482

Intramedullary Fixation of Diaphyseal Clavicle Fractures Using the Rockwood Clavicle Pin: Review of 86 Cases
William J Marlow*
Peter Ralte
Syam PK Morapudi
Rashpal Bassi
Jochen Fischer
Mohammad Waseem
Department of Trauma & Orthopaedics, Macclesfield District General Hospital, Victoria Road, Macclesfield, SK10 3BL, UK
*Address correspondence to this author at the Department of Trauma & Orthopaedics, Macclesfield District General Hospital, Victoria Road, Macclesfield, SK10 3BL, UK; Tel: 01625 66 13 15; E-mail: wjmarlow@gmail.com

INTRODUCTION

Fractures of the clavicle are common, accounting for 2.6% to 4% of adult fractures and 35% of injuries to the shoulder girdle [1-3]. Fractures of the shaft account for between 69% and 82% of all clavicle fractures [1, 3]. Undisplaced fractures of the clavicle shaft often heal with good clinical outcomes following non-operative management and there is general consensus that these should be treated non-operatively [4]. Outcomes following non-operative management of displaced mid-clavicular fractures are far less favourable than was once perceived, with several studies observing increased rates of non-union and symptomatic malunion [5-8]. A meta-analysis of articles addressing the treatment of displaced mid-clavicular fractures between 1975 and 2005 revealed a 15.1% non-union rate for closed treatment, 2.2% with compression plating, and 2% with intramedullary fixation [8]. Residual disability as measured by deficits in shoulder strength and endurance, as well as reduced patient satisfaction have also been observed in non-operatively treated displaced clavicle shaft fractures [9]. A recent multicenter randomised prospective clinical trial has shown improved functional outcome, lower rates of non-union and malunion following operative treatment of displaced clavicle fractures [10]. Factors associated with non-union and the development of long term sequelae following non-operative management of mid-clavicular fractures include displacement (in particular shortening of > 2 cm), comminution and advancing age [8]. Internal fixation of clavicle fractures is most commonly performed with plating. Intramedullary fixation has been previously described using Knowles pins [11, 12], Kirschner wires [13], Hagie pins [14] and elastic intramedullary nails [15-17]. While fixation with a plate involves a significant scar over the fracture area to allow placement of screws along the plate’s length, intramedullary fixation may be achieved with a much smaller scar at the fracture site and a second scar posterolaterally. The key evolutionary step between the Rockwood pin and previous intramedullary devices is that the threaded medial end and lateral locking nut allow both compression at the fracture site and resistance to migration of the metalwork. The purpose of this paper is to share the results of our experience with the Rockwood clavicle pin (DePuy, Warsaw, Indiana) in the treatment of acute diaphyseal clavicle fractures and non-union as an alternative to plate fixation.


MATERIALS AND METHODOLOGY

All cases of middle third clavicle fracture or non-union seen at one centre between February 2004 and February 2011 were included in the study. Of the 1086 cases, 985 patients were treated conservatively, 86 (7.9%) were treated by intramedullary fixation using the Rockwood clavicle pin and 15 (1.4%) underwent fixation with a plate. Indications for fixation were gross displacement of the fracture causing tenting of the skin, an open fracture or shortening by more than 20mm. Plate fixation was used in cases where the fracture extended proximally along the clavicle preventing the threaded medial end of the pin from gaining purchase, or distally preventing the locking nut from compressing the construct.

Of the 86 patients treated by Rockwood pin fixation, 70 (81.4%) underwent Rockwood pin fixation acutely or sub-acutely within 16 weeks of the injury (group A). Cases treated subsequent to the 16 week point were considered as surgery for symptomatic non- or mal-union since union would be expected by this time (group B). The mean time to surgery was 18 (2-64) days in group A and 28.3 (4.0-240.7) weeks in group B. Delays to surgery in group A were the result of late presentation e.g. injury abroad, failed initial non-operative management due to subsequent fracture displacement, or polytrauma requiring initial treatment of more serious injuries.

Higher numbers of males underwent fixation in both groups – 53 vs 17 in group A and 13 vs 3 in group B. The mean age was similar for each group – 34.4 in the group A and 38.1 in group B. The dominant side was injured in around half of cases in each group. Fractures were classified according to the Edinburgh system [3]. Two raters (WJM & PR) independently scored the radiographs and in the case of discrepancy, the senior author (MW) rated the radiograph for a third time. The most common subtype was 2B1 (Fig. 1A). In the group of patients treated by Rockwood pin fixation, the proportion of each sub-classification of fracture was similar to that seen in the group treated by plate fixation. 18 segmental 2B2 fractures were observed of which 17 were treated by Rockwood pin (Table 1).

The mechanism of injury was varied with the highest proportion of patients in the acute group sustaining injuries from road traffic collision (RTC), particularly involving a motorcycle or bicycle. A similar range of mechanisms caused the injuries in the group B (Table 2).

All procedures were performed by three Consultant orthopaedic surgeons specialising in upper limb trauma, with the majority of cases (79, 92%) performed by the senior author.

Case notes and radiographs were retrospectively reviewed to facilitate fracture classification and assess the incidence of complications. Measurements of pre and post-operative clavicle lengths were conducted using the Picture Archiving and Communication System (PACS) to assess for correction of shortening using the following method. Post-operative radiographs were standardised using the known diameter of the pin used in that patient. True post-operative length of the clavicle was then calculated. A bony landmark was then measured on both the pre-and post-operative films and the pre-operative film was standardised to the post-operative one. The true pre-operative length was calculated. If there was rotational difference between films, length could not be calculated. Functional outcome and patient satisfaction were measured by means of the Disability of the Arm, Shoulder and Hand (DASH) outcome measure [18] and an overall procedure related satisfaction questionnaire. Significance of correction of shortening and difference in length of stay was analysed using Student’s T-test. Statistics were calculated using Excel (Microsoft, WA USA).

Surgical Technique

The Rockwood clavicle pin was inserted as described in the manufacturer’s technique manual [19]. Patients were placed in the beach chair position and following sterile draping, a 3cm skin incision was made over the fracture site parallel to Langer’s lines. The fracture was exposed by dividing the fibres of the platysma taking care to avoid injury to the middle branch of the supraclavicular nerve which usually lies just below it. Towel clips were used to grasp the medial and lateral fragments which were then sequentially drilled and tapped. The Rockwood pin was inserted antegrade into the lateral fragment using a Jacobs chuck and T-handle. The pin was then passed through the posterior cortex of the lateral fragment and exited through the skin via a small stab incision where it was retrieved laterally. Once the pin had retracted sufficiently into the lateral fragment, the fracture was reduced and the pin was passed retrogradely into the intramedullary canal of the medial fragment. Finally, two locking nuts were fitted to compress the fracture if needed, and to secure the pin laterally (Fig. 2). The Rockwood pins used in each group were of a similar size of median 3.8mm. Allomatrix Injectable Putty (Wright Medical Technology, Arlington, TN) was used in 20% of acute fractures in the presence of significant comminution and in 87.5% of cases in group B. The pin was then trimmed below the skin, and both incisions were sutured. All patients received prophylactic intravenous antibiotics at induction. Postoperatively patients were placed in a sling for 2 weeks. They were then instructed to resume activities of daily living as tolerated but avoid strenuous activities such as pulling, lifting or pushing and arm elevation higher than face level for 4 to 6 weeks. Patients were routinely listed for removal of Rockwood pin when there was clinical and radiological evidence of union 16 weeks post-operatively. Radiographic union was defined as the appearance of bridging callous. If there was evidence of delayed union, removal of the pin was postponed.


RESULTS

In group A, the pins were removed at a mean of 3.6 months and follow-up was for a mean of 7.1 months. Functional assessments and satisfaction measures were carried out at a mean of 45 months. In group B, the pins were removed at a mean of 5.5 months and follow-up was for a mean of 8.7 months. Functional assessments and satisfaction measures were carried out at a mean of 34 months.

Sixty-eight fractures in group A went on to unite (Fig. 1A-C). The two cases of non-union were revised using a contoured locking plate and bone graft. There were no cases of non union in the group B although one case had not united at 8 months when the pin was removed – this was treated expectantly and united after a further 18 months (Table 3).

The predominant complication in both groups was posterior pin prominence causing significant irritation which resulted in wound oozing with or without superficial infection in several of the cases. Consequently four wounds in the acute group broke down exposing metalwork. Two of these underwent trimming at 14 and 48 days following pin insertion. The remaining two cases were managed expectantly with regular wound dressing until fracture union and pin removal. In all cases, symptoms of irritation resolved following hardware removal (Table 3).

One intra-operative complication was encountered which involved fracture of a drill tip while drilling the medial fragment. The broken tip was left in situ and due to its small size, no difficulties were encountered introducing or removing the pin following fracture union (Table 3).

The pre-operative shortening was corrected in the post-operative films by 8mm in group A and 6mm in group B. This was statistically significant in group A and showed a strong trend in group B (Table 4).

A reasonable response to DASH and satisfaction questionnaires was obtained – 71% in group A and 81% in group B. The mean DASH score was similar for both groups: 5.9 for group A and 8.7 for group B. All responders said they would have the procedure again and, although the vast majority of the acute group rated their satisfaction as good or excellent, 7.7% of group B rated their outcome as fair (Table 5).


DISCUSSION
Union

2 cases of non-union were identified in group A. - one was at the start of the series and one four years later. The cases were performed at one and four weeks post injury. The rate of non-union of 2.9% in the acute fracture group is slightly lower than that previously seen for other intramedullary fixation (4.7%) [20-25]. This rate is similar to the rate reported for plating of acute fractures 2.2% [8]. There were no episodes of malunion in this series, however, Mirzatolooei reported an incidence of 14% in plate fixation of acute fractures [26]. There were no cases of non-union in group B, reflecting a previous study by Lee [27], although there was one case of delayed union. In this case the clavicle was lengthened by 36mm (35%) and it is likely that the bone gap produced by this lengthening took longer to heal. A rate of 6.8% is seen following plating for non-union [28] possibly due to stripping of periosteum. This 100% rate of union in delayed fixation is promising; however the numbers in this study are too small to make a firm conclusion based on this.

Complications

There does appear to be a high rate of pin prominence and irritation for both acute and delayed groups, although, it should be noted that around 53% of patients with plate fixation go on to require plate removal for irritation, infection or prominence [22]. The 17.1% rate of irritation from pin prominence is higher than that seen by Kettler from fixation with elastic intramedullary nails (4.6%) [16], however an equal number of elastic nails required early removal due to migration; migration was not seen with this device. Migration of elastic nails required secondary shortening in 16.1% of cases in a study by Mueller [15]. Hagie pins have been associated with an 18.8% rate of wound breakdown requiring debridement [14] which is higher than that seen in our study. The Rockwood pin may cause irritation if not cut sufficiently short, however its design appears to have reduced the risk of migration and thereby the risk of protrusion through the skin or into important structures in the vicinity [29].

Pin prominence was generally described by patients as a “mild to moderate irritation” and no patients required expedited pin removal on account of this. It should be noted that these symptoms universally resolved after hardware removal. All four cases of metalwork exposure and the majority of cases of pin prominence occurred before December 2006. This reduction in posterior pin prominence and metalwork exposure over the series was thought to be due to the introduction of a purpose-designed “Rockwood pin cutter” allowing the pin to be cut more flush to the bolts and modification of the surgical technique so that the pin exited the lateral end of the clavicle in a more inferior position, thus providing additional soft tissue coverage for the pin.

In this study, patients were mobilised at an early stage and returned to full activities at 4-6 weeks. There was no evidence of hardware failure or fatigue (seen with elastic nails [15]), even with 2.5mm diameter pins.

Re-fracture following implant removal [30] and neurovascular and pulmonary injury from over drilling of the clavicle [31] have been reported in plate fixation, however these complications are avoided when using intramedullary fixation.

Satisfaction

DASH and satisfaction scores were generally good in both groups despite the high rate of complications. This probably reflects the fact that the majority of complications were caused by the pressure effect of a prominent pin and these resolved in all cases once the pin was removed. The DASH scores seen in this series were similar to those seen in patients treated by plate fixation [26, 32].

Cosmesis is an important factor, particularly in females. The Rockwood pin can be introduced through a 3cm incision at the level of the fracture site, rather than a 6cm incision required for a plate. This less invasive approach also reduces the risk to the supraclavicular nerve [33]. In the event of non-union requiring revision plating, the scar could be simply extended along Langer’s lines although this series demonstrates this is an uncommon requirement.

Removal of metalwork is required in all cases of intramedullary fixation compared to only half of the cases where the fracture is plated [22]. This is a key disadvantage of this technique, however while removal of a plate requires a general anaesthetic, the pin could potentially be removed under local anaesthetic in the clinic setting.

Correction of Shortening

A mean correction of shortening of 8mm was demonstrated in group A when the pre- and post-operative films were compared which, although small, was statistically significant. The mean clavicle lengthening was smaller and non-significant in group B and this reflects the fact that several of the cases had previously been reduced with an attempt at fixation.


LIMITATIONS

Shortcomings of our study include the relatively small number of procedures performed and the short follow-up period. The retrospective nature of data collection, incompleteness of the functional outcome scores obtained and the lack of a control for comparison are also acknowledged. Despite this, our series is the largest of its kind and gives a robust picture of patient centred and clinical outcomes when using Rockwood clavicle pins.


CONCLUSION

Complications can be reduced by using purpose-designed Rockwood pin cutter, the use of bone graft in comminuted fractures and non-union, ensuring the lateral pin exit point is inferior and that the correction in shortening does not leave a void. There does appear to be a higher rate of irritation symptoms in patients with a slim build.

Rockwood pins appear to be as effective as plates in achieving union and maintaining length in both the acute and delayed setting with the advantages of smaller skin incisions and less periosteal disruption than a plate. Although they seemingly avoid the potentially catastrophic risks of migration seen in other intramedullary devices, the rate of pin prominence and need for a further procedure present a significant disadvantage. The 100% union rate in the delayed fixation group is promising, however the numbers in this group were small and an extended study is required to validate this. In choosing between plate and Rockwood pin fixation, one should weigh this less invasive technique against its potential for inconvenience and complications.


ACKNOWLEDGEMENTS

The authors listed are responsible for production of this work from conception to final copy.

CONFLICT OF INTEREST

There were no financial contributions to the work reported. The authors have no conflict of interest to declare.


ABBREVIATIONS
DASH  = Disability of the Arm, Shoulder and Hand
PACS  = Picture Archiving and Communication System
RTC  = Road Traffic Collision

REFERENCES
1. Postacchini F,Gumina S,De Santis P,Albo F. Epidemiology of clavicle fracturesJ Shoulder Elbow SurgYear: 2002115 452612378163
2. Nordqvist A,Petersson C. The incidence of fractures of the clavicleClin Orthop Relat ResYear: 1994300127328131324
3. Robinson CM. Fractures of the clavicle in the adultEpidemiology and classification. J Bone Joint Surg BrYear: 1998803 47684
4. Khan LA,Bradnock TJ,Scott C,Robinson CM. Fractures of the clavicleJ Bone Joint Surg AmYear: 2009912 4476019181992
5. Robinson CM,Court-Brown CM,McQueen MM,Wakefield AE. Estimating the risk of nonunion following nonoperative treatment of a clavicular fractureJ Bone Joint Surg AmYear: 200486-A7 13596515252081
6. Hill JM,McGuire MH,Crosby LA. Closed treatment of displaced middle-third fractures of the clavicle gives poor resultsJ Bone Joint Surg BrYear: 1997794 53799250733
7. McKee MD,Wild LM,Schemitsch EH. Midshaft malunions of the clavicleJ Bone Joint Surg AmYear: 200385-A5 790712728026
8. Zlowodzki M,Zelle BA,Cole PA,Jeray K,McKee MD. Treatment of acute midshaft clavicle fractures: systematic review of 2144 fractures: on behalf of the Evidence-Based Orthopaedic Trauma Working GroupJ Orthop TraumaYear: 2005197 504716056089
9. McKee MD,Pedersen EM,Jones C,et al. Deficits following nonoperative treatment of displaced midshaft clavicular fracturesJ Bone Joint Surg AmYear: 2006881 354016391247
10. Canadian Orthopaedic Trauma Society. Nonoperative treatment compared with plate fixation of displaced midshaft clavicular fractures. A multicenter randomized clinical trialJ Bone Joint Surg AmYear: 2007891 110
11. Zenni EJ Jr,Krieg JK,Rosen MJ. Open reduction and internal fixation of clavicular fracturesJ Bone Joint Surg AmYear: 1981631 147517451517
12. Neviaser RJ,Neviaser JS,Neviaser TJ. A simple technique for internal fixation of the clavicle. A long term evaluationClin Orthop Relat ResYear: 197510910371132190
13. Ngarmukos C,Parkpian V,Patradul A. Fixation of fractures of the midshaft of the clavicle with Kirschner wires Results in 108 patientsJ Bone Joint Surg BrYear: 1998801 10689460963
14. Strauss EJ,Egol KA,France MA,Koval KJ,Zuckerman JD. Complications of intramedullary Hagie pin fixation for acute midshaft clavicle fracturesJ Shoulder Elbow SurgYear: 2007163 280417363289
15. Mueller M,Rangger C,Striepens N,Burger C. Minimally invasive intramedullary nailing of midshaft clavicular fractures using titanium elastic nailsJ TraumaYear: 2008646 15283418545118
16. Kettler M,Schieker M,Braunstein V,Konig M,Mutschler W. Flexible intramedullary nailing for stabilization of displaced midshaft clavicle fractures technique and results in 87 patients Acta OrthopYear: 2007783 424917611859
17. Jubel A,Andermahr J,Schiffer G,Rehm KE. Technique of intramedullary osteosynthesis of the clavicle with elastic titanium nailsUnfallchirurgYear: 20021056 511612132189
18. Hudak PL,Amadio PC,Bombardier C. Development of an upper extremity outcome measure: the DASH (disabilities of the arm shoulder and hand) [corrected] The Upper Extremity Collaborative Group (UECG)Am J Ind MedYear: 1996296 60288773720
19. DePuy International Ltd. Rockwood clavicle pin: design rationale and surgical techniqueYear: 2005110
20. Chen YF,Zeng BF,Chen YJ,et al. Clinical outcomes of midclavicular fractures treated with titanium elastic nailsCan J SurgYear: 2010536 3798421092430
21. Bohme J,Bonk A,Bacher GO,Wilharm A,Hoffmann R,Josten C. Current treatment concepts for mid-shaft fractures of the clavicle - results of a prospective multicentre studyZ Orthop UnfallYear: 20111491 687620941694
22. Ferran NA,Hodgson P,Vannet N,Williams R,Evans RO. Locked intramedullary fixation vs plating for displaced and shortened midshaft clavicle fractures: a randomized clinical trialJ Shoulder Elbow SurgYear: 2010196 783920713274
23. Kleweno CP,Jawa A,Wells JH,et al. Midshaft clavicular fractures: comparison of intramedullary pin and plate fixationJ Shoulder Elbow SurgYear: 2011207 1114721723149
24. Mudd CD,Quigley KJ,Gross LB. Excessive Complications of Open Intramedullary Nailing of Midshaft Clavicle Fractures With the Rockwood Clavicle PinClin Orthop Relat ResYear: 201146933647021424833
25. Millett PJ,Hurst JM,Horan MP,Hawkins RJ. Complications of clavicle fractures treated with intramedullary fixationJ Shoulder Elbow SurgYear: 2011201 869121050778
26. Mirzatolooei F. Comparison between operative and nonoperative treatment methods in the management of comminuted fractures of the clavicleActa Orthop Traumatol TurcYear: 2011451 344021478660
27. Lee YS,Lin CC,Huang CR,Chen CN,Liao WY. Operative treatment of midclavicular fractures in 62 elderly patients: knowles pin versus plateOrthopedicsYear: 20073011 9596418019991
28. Shen JW,Tong PJ,Qu HB. A three-dimensional reconstruction plate for displaced midshaft fractures of the clavicleJ Bone Joint Surg BrYear: 20089011 1495818978272
29. Sethi GK,Scott SM. Subclavian artery laceration due to migration of a Hagie pinSurgeryYear: 19768056446790622
30. Poigenfurst J,Rappold G,Fischer W. Plating of fresh clavicular fractures: results of 122 operationsInjuryYear: 1992234 237411618563
31. Shackford SR,Connolly JF. Taming of the screw: a case report and literature review of limb-threatening complications after plate osteosynthesis of a clavicular nonunionJ TraumaYear: 2003555 840314608153
32. Kabak S,Halici M,Tuncel M,Avsarogullari L,Karaoglu S. Treatment of midclavicular nonunion: comparison of dynamic compression plating and low-contact dynamic compression plating techniquesJ Shoulder Elbow SurgYear: 2004134 39640315220879
33. Wang K,Dowrick A,Choi J,Rahim R,Edwards E. Post-operative numbness and patient satisfaction following plate fixation of clavicular fracturesInjuryYear: 20104110 1002520219192

Article Categories:
  • Article

Keywords: Keywords Clavicle [A02.835.232.087.227], Fracture Fixation, Intramedullary [E04.555.300.300.300], Fracture Healing [G16.100.856.891.500], Fractures, Malunited [C26.404.249], Fractures, Ununited [C26.404.468], Rockwood Clavicle Pin, Surgical Procedures, Minimally Invasive [E04.800]..

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