Document Detail

Intraluminal middle cerebral artery occlusion (MCAO) model for ischemic stroke with laser doppler flowmetry guidance in mice.
MedLine Citation:
PMID:  21587164     Owner:  NLM     Status:  MEDLINE    
Stroke is the third leading cause of death and the leading cause of disability in the world, with an estimated cost of near $70 billion in the United States in 2009. The intraluminal middle cerebral artery occlusion (MCAO) model was developed by Koizumi in 1986 to simulate this impactful human pathology in the rat. A modification of the MCAO method was later presented by Longa. Both techniques have been widely used to identify molecular mechanisms of brain injury resulting from ischemic stroke and potential therapeutic modalities. This relatively noninvasive method in rats has been extended to use in mice to take advantage of transgenic and knockout strains. To model focal cerebral ischemia, an intraluminal suture is advanced via the internal carotid artery to occlude the base of the MCA. Retracting the suture after a specified period of time mimics spontaneous reperfusion, but the suture can also be permanently retained. This video will be demonstrating the two major approaches for performing intraluminal MCAO procedure in mice in a stepwise fashion, as well as providing insights for potential drawbacks and pitfalls. The ischemic brain tissue will subsequently be stained by 2,3,5-triphenyltetrazolium chloride (TTC) to evaluate the extent of cerebral infarction.
Saeed Ansari; Hassan Azari; Douglas J McConnell; Aqeela Afzal; J Mocco
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Publication Detail:
Type:  Journal Article; Video-Audio Media     Date:  2011-05-08
Journal Detail:
Title:  Journal of visualized experiments : JoVE     Volume:  -     ISSN:  1940-087X     ISO Abbreviation:  J Vis Exp     Publication Date:  2011  
Date Detail:
Created Date:  2011-05-18     Completed Date:  2011-08-26     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101313252     Medline TA:  J Vis Exp     Country:  United States    
Other Details:
Languages:  eng     Pagination:  -     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Journal of Visualized Experiments
Department of Neurosurgery, University of Florida, USA.
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MeSH Terms
Disease Models, Animal*
Infarction, Middle Cerebral Artery / etiology*
Laser-Doppler Flowmetry / methods*
Stroke / etiology*

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