Document Detail

Intrahepatic transfusion-transmitted virus detected by in situ hybridization in patients with liver diseases.
MedLine Citation:
PMID:  10886539     Owner:  NLM     Status:  MEDLINE    
Transfusion-transmitted virus (TTV) has been identified from patients with post-transfusion hepatitis of unknown aetiology, but the clinical relevance remains unclear. The aim of this study was to evaluate TTV in liver. We studied 15 patients with hepatitis non-A-E and 44 with hepatitis B virus (HBV). The nested polymerase chain reaction (PCR) with primers corresponding to the conserved region of the published TTV genome was employed to amplify TTV fragments in serum, and in situ hybridization was used to detect TTV in biopsied liver specimens. TTV DNA was detected in serum from six (40%) of 15 patients with hepatitis of unknown aetiology and from 16 (36.4%) of 44 patients with chronic hepatitis B, respectively. The intrahepatic viral fragment was detected in 17 (77.3%) of 22 patients with TTV in serum. There was no statistical difference in the prevalence of TTV infection between the two groups (hepatitis non-A-E 40% vs HBV 25%, P > 0.75). When patients in both groups, with and without TTV, were compared, no differences were found in serum alanine aminotransferase (ALT) levels (hepatitis non-A-E: 131.5 +/- 66.6 vs 244.2 +/- 257.4, P=0.955; HBV: 240.1 +/- 418.9 vs 214.6 +/- 276.7 U l(-1), P=0.761) or histological activity index (grade) score (hepatitis non-A-E: 6.4 +/- 5.5 vs 5.6 +/- 5.9, P=0.689; HBV: 5.6 +/- 3.7 vs 5.5 +/- 3.7, P=0.345). HBV DNA levels in patients with and without TTV co-infection did not differ significantly (300 +/- 776.4 microg ml(-1) vs 97.1 +/- 160.5 microg ml(-1), P=0.980). Hence, TTV does exist in liver, but plays no role in hepatitis or aggravation of liver damage when co-infected with HBV.
X J Jiang; K X Luo; H T He
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of viral hepatitis     Volume:  7     ISSN:  1352-0504     ISO Abbreviation:  J. Viral Hepat.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-09-29     Completed Date:  2000-09-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9435672     Medline TA:  J Viral Hepat     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  292-6     Citation Subset:  IM    
Department of Infectious Disease, Nanfang Hospital, Guangzhou, China.
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MeSH Terms
Base Sequence
Blood Transfusion / adverse effects*
DNA Primers / genetics
DNA, Viral / blood,  genetics,  isolation & purification
Hepatitis B / virology
Hepatitis Viruses / genetics,  isolation & purification*
Hepatitis, Viral, Human / pathology,  transmission*,  virology*
In Situ Hybridization
Liver / pathology,  virology*
Middle Aged
Reg. No./Substance:
0/DNA Primers; 0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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