Document Detail


Intracytoplasmic morphologically selected sperm injection improves development and quality of preimplantation embryos in teratozoospermia patients.
MedLine Citation:
PMID:  22717245     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This prospective randomized study investigated whether intracytoplasmic sperm injection (ICSI) outcome can be improved with sperm preselection under ×6000 magnification and intracytoplasmic morphologically selected sperm injection (IMSI) in patients with teratozoospermia and characterized embryo development and quality regarding sperm morphology and presence of head vacuoles. Couples with isolated teratozoospermia were divided into two groups: IMSI group (n=52) and ICSI group (n=70) and fertilization, blastocyst and clinical pregnancy rates were compared. Oocytes from 30 randomly chosen patients from the IMSI group were injected with spermatozoa that had been previously classified under ×6000 magnification into four classes according to the number and size of vacuoles in the head and then cultured separately. Pronuclear morphology, embryo development and blastomere viability were estimated to investigate the influence of sperm morphology, especially vacuoles, on embryo developmental capacity. A significantly higher clinical pregnancy rate was achieved in the IMSI group compared with the ICSI group (48% versus 24%, P<0.05). Fertilization with spermatozoa without head vacuoles yielded higher number of morphologically normal zygotes, higher blastocyst rate and smaller proportion of arrested embryos than spermatozoa with vacuoles and other head defects. IMSI is a method of choice in patients with teratozoospermia. Data suggests that morphologically abnormal spermatozoa and/or spermatozoa with fragmented DNA have a negative impact on fertilization and embryo quality, even when intracytoplasmic sperm injection (ICSI) is performed. The aim of our prospective randomized study was to see if an ICSI outcome can be improved with sperm pre-selection under ×6000 magnification (intracytoplasmic morphologically selected sperm injection, IMSI) in patients with teratozoospermia and to characterize embryo development and quality regarding the sperm morphology and the presence of head vacuoles. Couples with isolated teratozoospermia were divided into two groups: IMSI group (n=52) and ICSI group (n=70). Fertilization, blastocyst and pregnancy rates were compared between the two groups. Oocytes from 30 randomly chosen patients from the IMSI group were injected with spermatozoa that had been previously classified under ×6000 magnification into four classes according to the number and size of vacuoles in the head and then cultured separately. Pronuclear morphology, embryo development and blastomere viability were estimated in order to see the influence of sperm morphology, especially vacuoles, on embryo developmental capacity. A statistically significant higher pregnancy rate was achieved in the IMSI group compared with the ICSI group (48% versus 24%, P<0.05). Fertilization with spermatozoa without head vacuoles yielded a higher number of morphologically normal zygotes, higher blastocyst rate and smaller proportion of arrested embryos than spermatozoa with vacuoles and other head defects. This means that IMSI is a method of choice in patients with teratozoospermia.
Authors:
Katja Knez; Tomaz Tomazevic; Branko Zorn; Eda Vrtacnik-Bokal; Irma Virant-Klun
Related Documents :
9885025 - Sonographically detected fetal and placental abnormalities associated with trisomy 16 c...
8559755 - Identification of a case of maternal uniparental disomy of chromosome 10 associated wit...
12124685 - Prenatal diagnosis of congenital heart disease in the naples area during the years 1994...
8178865 - Androgenesis and gynogenesis are not causative in early pregnancy loss in humans.
12548225 - Increase in maternal placental growth hormone during pregnancy and disappearance during...
11682935 - A preliminary assessment of the potential of the muschovy duck (cairina maschata) as a ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-3
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  -     ISSN:  1472-6491     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-6-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
Affiliation:
Reproductive Unit, Department of Obstetrics and Gynaecology, University Medical Centre Ljubljana, Ljubljana, Slovenia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Adaptive Breast Radiation Therapy Using Modeling of Tissue Mechanics: A Breast Tissue Segmentation S...
Next Document:  DHEA for poor responders: can treatment be justified in the absence of evidence?