Document Detail


Intracytoplasmic epidermal growth factor receptor shows poor response to the cetuximab antitumor effect in irradiated non-small cell lung cancer cell lines.
MedLine Citation:
PMID:  22704525     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The purpose of this study was to evaluate the antitumor effect of a blocking antibody for EGFR in the cytoplasm of a lung cancer cell line.
METHODS & MATERIALS: The A549 and H1299 cell lines were employed to demonstrate differential responses to cetuximab in combination with radiotherapy. Localization of EGFR was detected using confocal microscopy, and radiosensitivity was measured.
RESULTS: Treatment with cetuximab inhibited colony formation in a dose-dependent manner in A549, but not H1299 cells. Confocal microscopy revealed EGFR localized in the cytosolic fraction, particularly around the golgi apparatus in H1299, in contrast to cell membrane localization in A549 cell line. After irradiation, nuclear EGFR was detected in the A549 cell line. However, EGFR did not translocate to the nucleus in H1299 cells. While EGFR expression was decreased in both A549 and H1299 cell lines upon treatment with a combination of cetuximab and radiation, radiosensitivity was increased solely in A549 cells.
CONCLUSIONS: Our findings suggest that localization of EGFR is related to the sensitivity/resistance of cells to a combination of cetuximab and radiotherapy.
Authors:
Seung-Hee Ryu; Sang-Wook Lee; Youn-Joo Yang; Si Yeol Song; Jong Hoon Kim; Eun Kyung Choi; Seung Do Ahn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-14
Journal Detail:
Title:  Lung cancer (Amsterdam, Netherlands)     Volume:  77     ISSN:  1872-8332     ISO Abbreviation:  Lung Cancer     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-20     Completed Date:  2012-12-21     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  8800805     Medline TA:  Lung Cancer     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  482-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Department of Radiation Oncology, University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / pharmacology*
Antineoplastic Agents / pharmacology*
Carcinoma, Non-Small-Cell Lung / drug therapy*,  radiotherapy
Cell Line, Tumor / drug effects,  radiation effects
Cell Survival / drug effects,  radiation effects
Chemoradiotherapy
Cytoplasm / drug effects,  metabolism*
Drug Resistance, Neoplasm
Humans
Lung Neoplasms / drug therapy*,  radiotherapy
Protein Transport / drug effects,  radiation effects
Radiation Tolerance
Receptor, Epidermal Growth Factor / antagonists & inhibitors,  metabolism*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antineoplastic Agents; EC 2.7.10.1/Receptor, Epidermal Growth Factor; PQX0D8J21J/cetuximab

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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