Document Detail


Intracranial stereotaxic cannulation for development of orthotopic glioblastoma allograft in Sprague-Dawley rats and histoimmunopathological characterization of the brain tumor.
MedLine Citation:
PMID:  17701349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glioblastoma is the most common brain tumor that causes significant mortality annually. Limitations of the current therapeutic regimens warrant development of new techniques and treatment strategies in orthotopic animal model for better management of this devastating brain cancer. There are only a few experimental orthotopic models of glioblastoma for pre-clinical testing. In the present investigation, we successfully implanted rat C6 cells via intracranial stereotaxic cannulation in adult Sprague-Dawley rats for development and histoimmunopathological characterization of an advanced orthotopic glioblastoma allograft model, which could be useful for investigating the course of glioblastoma development as well as for testing efficacy of new therapeutic agents. The orthotopic glioblastoma allograft was generated by intracerebral injection of rat C6 cells through a guide-cannula system and after 21 post-inoculation days the brain tumor was characterized by histoimmunopathological experiments. Histological staining and immunofluorescent labelings for TERT, VEGF, Bcl-2, survivin, XIAP, and GFAP revealed the distinct characteristics of glioblastoma in C6 allograft, which could be useful as a target for treatment with emerging new therapeutic agents. Our investigation indicated the successful development of intracranial cannulated orthotopic glioblastoma allograft in adult Sprague-Dawley rats, making it as a useful animal model of glioblastoma for pre-clinical evaluation of various therapeutic strategies for the management of glioblastoma.
Authors:
Surajit Karmakar; M Foster Olive; Naren L Banik; Swapan K Ray
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-08-15
Journal Detail:
Title:  Neurochemical research     Volume:  32     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-02     Completed Date:  2008-01-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2235-42     Citation Subset:  IM    
Affiliation:
Department of Neurosciences, Medical University of South Carolina, Charleston, SC 29425, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Neoplasms / pathology*
Catheterization / methods
Cell Transplantation / instrumentation,  methods
Coloring Agents
Fluorescent Antibody Technique
Glioblastoma / pathology*
Immunohistochemistry
In Situ Hybridization
Male
Neoplasm Transplantation / instrumentation,  methods*
Rats
Rats, Sprague-Dawley
Stereotaxic Techniques
Tumor Markers, Biological
Grant Support
ID/Acronym/Agency:
C06 RR015455/RR/NCRR NIH HHS; NS-57811/NS/NINDS NIH HHS; R01 CA-91460/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Coloring Agents; 0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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