Document Detail


Intracoronary administration of cardiac stem cells in mice: a new, improved technique for cell therapy in murine models.
MedLine Citation:
PMID:  21516491     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A model of intracoronary stem cell delivery that enables transgenesis/gene targeting would be a powerful tool but is still lacking. To address this gap, we compared intracoronary and intramyocardial delivery of lin(-)/c-kit(+)/GFP(+) cardiac stem cells (CSCs) in a murine model of reperfused myocardial infarction (MI). Lin(-)/c-kit(+)/GFP(+) CSCs were successfully expanded from GFP transgenic hearts and cultured with no detectable phenotypic change for up to ten passages. Intracoronary delivery of CSCs 2 days post-MI resulted in significant alleviation of adverse LV remodeling and dysfunction, which was at least equivalent, if not superior, to that achieved with intramyocardial delivery. Compared with intramyocardial injection, intracoronary infusion was associated with a more homogeneous distribution of CSCs in the infarcted region and a greater increase in viable tissue in this region, suggesting greater formation of new cardiomyocytes. Intracoronary CSC delivery resulted in improved function in the infarcted region, as well as in improved global LV systolic and diastolic function, and in decreased LV dilation and LV expansion index; the magnitude of these effects was similar to that observed after intramyocardial injection. We conclude that, in the murine model of reperfused MI, intracoronary CSC infusion is at least as effective as intramyocardial injection in limiting LV remodeling and improving both regional and global LV function. The intracoronary route appears to be superior in terms of uniformity of cell distribution, myocyte regeneration, and amount of viable tissue in the risk region. To our knowledge, this is the first study to report that intracoronary infusion of stem cells in mice is feasible and effective.
Authors:
Qianhong Li; Yiru Guo; Qinghui Ou; Ning Chen; Wen-Jian Wu; Fangping Yuan; Erin O'Brien; Tao Wang; Li Luo; Gregory N Hunt; Xiaoping Zhu; Roberto Bolli
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-04-24
Journal Detail:
Title:  Basic research in cardiology     Volume:  106     ISSN:  1435-1803     ISO Abbreviation:  Basic Res. Cardiol.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-04     Completed Date:  2012-01-18     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0360342     Medline TA:  Basic Res Cardiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  849-64     Citation Subset:  IM    
Affiliation:
Division of Cardiovascular Medicine, University of Louisville, 550 S. Jackson Street, Louisville, KY 40292, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement / physiology
Cells, Cultured
Coronary Vessels*
Female
Green Fluorescent Proteins / metabolism
Injections, Intra-Arterial
Mice
Mice, Inbred C57BL
Mice, Transgenic
Models, Animal*
Myocardial Infarction / pathology,  therapy*
Myocardium / cytology*,  metabolism
Proto-Oncogene Proteins c-kit / metabolism
Regeneration / physiology
Stem Cells / cytology*,  metabolism
Tissue Therapy / methods*
Treatment Outcome
Grant Support
ID/Acronym/Agency:
HL-70897/HL/NHLBI NIH HHS; HL-76794/HL/NHLBI NIH HHS; P01 HL078825/HL/NHLBI NIH HHS; P01HL78825/HL/NHLBI NIH HHS; R01 HL055757/HL/NHLBI NIH HHS; R01 HL070897/HL/NHLBI NIH HHS; R01 HL076794/HL/NHLBI NIH HHS; R01 HL55757/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins; EC 2.7.10.1/Proto-Oncogene Proteins c-kit
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