Document Detail

Intracomplex general acid/base catalyzed cleavage of RNA phosphodiester bonds: the leaving group effect.
MedLine Citation:
PMID:  22825538     Owner:  NLM     Status:  Publisher    
The general acid/base catalyzed cleavage of a number of alkyl esters of uridine-3'- (and -5'-)phosphate has been studied by utilizing a cleaving agent, in which the catalytic moiety (a substituted 1,3,5-triazine) is tethered to an anchoring Zn(II):cyclen moiety. Around pH 7, formation of a strong ternary complex between uracil, Zn(II) and cyclen brings the general acid/base catalyst close to the scissile phosphodiester linkage, resulting in rate acceleration of 1-2 orders of magnitude with the uridine-3'-phosphodiesters. Curiously, no acceleration was observed with their 5'-counterparts. A β(lg) value of -0.7 has been determined for the general acid/base catalyzed cleavage, consistent with a proton transfer to the leaving group in the rate-limiting step.
Tuomas Lönnberg; Mikko Luomala
Related Documents :
8739188 - The occurrence of glycine in bacterial lipopolysaccharides.
18647178 - Effect of a carbon source on polyphosphate accumulation in saccharomyces cerevisiae.
12198488 - The structural basis for specificity in human abo(h) blood group biosynthesis.
5837778 - Properties of the polysaccharide and mucopeptide components of the cell wall of lactoba...
17408508 - Effect of carbon source perturbations on transcriptional regulation of metabolic fluxes...
7656298 - Effects of caffeine intake during gestation and lactation on the acid solubility of ena...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-24
Journal Detail:
Title:  Organic & biomolecular chemistry     Volume:  -     ISSN:  1477-0539     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101154995     Medline TA:  Org Biomol Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Chemistry, University of Turku, FIN-20014, Turku, Finland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Impact of Chronic Kidney Disease on Long-term Clinical Outcomes After Percutaneous Coronary Interven...
Next Document:  Upregulation of miR-20a and miR-106b is involved in the acquisition of malignancy of pediatric brain...