Document Detail

Intracisternal increase of superoxide anion production in a canine subarachnoid hemorrhage model.
MedLine Citation:
PMID:  11239179     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND PURPOSE: Reactive oxygen species (ROS) are thought to be primary in the pathogenesis of cerebral vasospasm after subarachnoid hemorrhage (SAH). However, as direct evidence of ROS has not yet been demonstrated in cerebral vasospasm, we sought to substantiate superoxide anion (.O(2)(-)) generation in the subarachnoid space after SAH using a modification of Karnovsky's manganese/diaminobenzidine (Mn(2+)/DAB) technique. METHODS: SAH or sham operation was induced according to a 2-hemorrhage model in a total of 24 beagle dogs. On day 2 or 7 after SAH or sham operation, dogs were intrathecally infused with buffer containing Mn(2+) and DAB, and the brain stem was prepared for light and electron microscopy. Possible colocalization of ferrous (Fe(2+)) or ferric (Fe(3+)) iron ions with.O(2)(-) was also examined with the use of Turnbull blue or Berlin blue staining, respectively. RESULTS: Light microscopy revealed amorphous, amber deposits within the subarachnoid hematoma, the periarterial space, and the tunica adventitia of the basilar artery on days 2 and 7 after SAH.O(2)(-) deposits were eliminated by addition of superoxide dismutase or exclusion of either Mn(2+) or DAB from the perfusate, confirming the specificity of the reaction. These deposits were colocalized with blue reaction deposits indicating Fe(2+) and Fe(3+). Within the subarachnoid space,.O(2)(-) indicating electron-dense fine granules were preferentially located around degenerated erythrocytes and, secondarily, infiltrating macrophages and neutrophils. CONCLUSIONS: We show direct evidence for enhanced production of.O(2)(-) and Fe(2+)/Fe(3+) iron ions in the subarachnoid space after SAH, lending further support to the pathogenic role of ROS in cerebral vasospasm after SAH.
T Mori; K Nagata; T Town; J Tan; T Matsui; T Asano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  32     ISSN:  1524-4628     ISO Abbreviation:  Stroke     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-12     Completed Date:  2001-04-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  United States    
Other Details:
Languages:  eng     Pagination:  636-42     Citation Subset:  IM    
Institute of Laboratory Animal Science and the Department of Neurosurgery, Saitama Medical Center/School, Saitama, Japan.
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MeSH Terms
3,3'-Diaminobenzidine / administration & dosage,  metabolism
Basilar Artery / pathology
Disease Models, Animal*
Disease Progression
Erythrocytes / pathology
Injections, Spinal
Iron / metabolism
Macrophages / pathology
Manganese / administration & dosage,  metabolism
Microscopy, Electron
Neutrophils / pathology
Subarachnoid Hemorrhage / metabolism*,  pathology
Subarachnoid Space / metabolism*,  pathology
Superoxides / metabolism*
Reg. No./Substance:
0/Ferrocyanides; 11062-77-4/Superoxides; 25869-98-1/potassium iron(III) hexacyanoferrate (II); 7439-89-6/Iron; 7439-96-5/Manganese; 91-95-2/3,3'-Diaminobenzidine

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