| Intrachromosomal recombination in plants. | |
| | |
MedLine Citation:
|
PMID: 2170114 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Molecular evidence for intrachromosomal recombination between closely linked DNA repeats within the plant genome is presented. The non-overlapping complementary deletion derivatives of the selectable neomycin phosphotransferase gene (nptII), when intact conferring kanamycin resistance, were inserted into the genome of Nicotiana tabacum. The functional marker gene was restored with frequencies between 10(-4) and 10(-6) per proliferating cell clone. Prolonged tissue culture prior to kanamycin selection did not increase the number of recombinant kanamycin-resistant (KanR) cell clones. DNA analysis of KanR clones derived from cells carrying multiple tandem recombination units suggested that these units have a tendency to undergo concerted recombination. Recovery and analysis of kanamycin-sensitive seedlings with patches of KanR cells provided direct evidence for mitotic recombination in plant tissue. |
| | |
Authors:
|
A Peterhans; H Schlüpmann; C Basse; J Paszkowski |
Related Documents
:
|
15493334 - Structure and function of the shufflon in plasmid r64. 11278954 - Triplex-induced recombination in human cell-free extracts. dependence on xpa and hsrad51. 14586554 - Gene disruption in trichoderma atroviride via agrobacterium-mediated transformation. 11426464 - System for efficient helper-dependent minimal adenovirus construction and rescue. 15493334 - Structure and function of the shufflon in plasmid r64. 22287484 - Xenopus white papers and resources: folding functional genomics and genetics into the f... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The EMBO journal Volume: 9 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 1990 Nov |
Date Detail:
|
Created Date: 1990-11-21 Completed Date: 1990-11-21 Revised Date: 2010-09-10 |
Medline Journal Info:
|
Nlm Unique ID: 8208664 Medline TA: EMBO J Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 3437-45 Citation Subset: IM |
Affiliation:
|
Institute for Plant Sciences, Swiss Federal Institute of Technology, ETH-Zürich, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Sequence Blotting, Southern Cell Line Chromosomes / physiology* Cloning, Molecular Kanamycin Kinase Meiosis Mitosis Molecular Sequence Data Phosphotransferases / genetics Plants, Toxic* Recombination, Genetic* Restriction Mapping Tobacco / genetics* |
| Chemical | |
Reg. No./Substance:
|
EC 2.7.-/Phosphotransferases; EC 2.7.1.95/Kanamycin Kinase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A specific DIF binding protein in Dictyostelium.
Next Document: Mannose 6-phosphate receptor dependent secretion of lysosomal enzymes.