Document Detail


Intracerebroventricular galanin-like peptide induces different brain activation compared with galanin.
MedLine Citation:
PMID:  12933672     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Like galanin, the 60-amino-acid peptide, galanin-like peptide (GALP), has orexigenic actions, demonstrated by an acute increase in feeding after central injection in rodents. However, in contrast to galanin, GALP causes a prolonged rise in core body temperature and a reduction in body weight over 24 h. In an attempt to identify potential explanations for the observed differences between GALP and galanin, this study examined which brain areas were activated by these peptides. Intracerebroventricular injection of GALP into conscious rats significantly stimulated feeding over 0-1 h, increased core body temperature, but reduced body weight gain over 24 h. Immunohistochemistry to detect c-fos demonstrated that intracerebroventricular injection of GALP or galanin activated several brain regions in common, including the dorsomedial nucleus of the hypothalamus, lateral hypothalamus, and nucleus tractus solitarius of the brainstem. However, GALP also induced c-fos expression in the periventricular hypothalamic region and supraoptic hypothalamic nucleus. Cell activation induced by GALP in the supraoptic hypothalamic nucleus and nucleus tractus solitarius was dependent on food intake but independent of food consumption in all other brain regions. Double immunohistochemistry indicated that small cells expressing c-fos in the periventricular hypothalamic region after GALP were astrocytes and not microglia.
Authors:
Catherine B Lawrence; Torrie Williams; Simon M Luckman
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  144     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-22     Completed Date:  2003-09-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3977-84     Citation Subset:  AIM; IM    
Affiliation:
School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / metabolism
Body Temperature / drug effects
Body Weight / drug effects
Eating / drug effects
Ependyma / cytology,  drug effects,  physiology
Galanin / pharmacology*
Galanin-Like Peptide
Hypothalamus, Anterior / cytology,  drug effects*,  physiology
Immunohistochemistry
Injections, Intraventricular
Male
Microglia / metabolism
Nerve Tissue Proteins / pharmacology*
Proto-Oncogene Proteins c-fos / metabolism
Rats
Rats, Sprague-Dawley
Solitary Nucleus / cytology,  drug effects,  physiology
Chemical
Reg. No./Substance:
0/Galanin-Like Peptide; 0/Nerve Tissue Proteins; 0/Proto-Oncogene Proteins c-fos; 88813-36-9/Galanin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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