Document Detail


Intracerebroventricular administration of apelin-13 inhibits distal colonic transit in mice.
MedLine Citation:
PMID:  20849897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apelin is a novel bioactive peptide as the endogenous ligand for the orphan G-protein-coupled receptor (GPCR), APJ, a receptor distributed in various tissues such as the hypothalamus and the gastrointestinal tract. Recent reports showed that apelin regulated many biological functions, including blood pressure, neuroendocrine, drinking behavior and food intake. However, the role of apelin in regulating gastrointestinal motility remains unknown. The present study aimed to investigate the actions of intracerebroventricularly administered apelin-13 on colonic transit as well as the actions of apelin-13 on the contraction of isolated distal colon in vitro. Intracerebroventricular (i.c.v.) injection of apelin-13 (0.3, 0.5, 1 and 3 μg/mouse) dose-dependently inhibited fecal pellet output and bead expulsion. This effect was significantly antagonized by the APJ receptor antagonist apelin-13(F13A), indicating an APJ receptor-mediated mechanism. Furthermore, naloxone could also reverse the inhibitory effect of apelin-13 on fecal pellet output and bead expulsion, suggesting the involvement of opioid receptors in the suppressive effect of apelin-13 on distal colon transit. However, apelin-13 (10⁻⁸-10⁻⁶ M) did not affect distal colonic contractions in vitro.
Authors:
Yan-Jie Yang; Shuang-Yu Lv; Ming-Hui Xiu; Ning Xu; Qiang Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-22
Journal Detail:
Title:  Peptides     Volume:  31     ISSN:  1873-5169     ISO Abbreviation:  Peptides     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-01     Completed Date:  2011-02-10     Revised Date:  2011-06-15    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2241-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Institute of Biochemistry and Molecular Biology, School of Life Science, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Colon / drug effects*
Feces
Gastrointestinal Transit / drug effects*
Injections, Intraventricular
Intercellular Signaling Peptides and Proteins / pharmacology*
Male
Mice
Muscle Contraction / drug effects
Muscle, Smooth / drug effects
Receptors, G-Protein-Coupled / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Aplnr protein, mouse; 0/Intercellular Signaling Peptides and Proteins; 0/Receptors, G-Protein-Coupled; 0/apelin-13 peptide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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