Document Detail

Intracellular regulation of endometrial PGF(2a) and PGE(2) production in dairy cows during early pregnancy and following treatment with recombinant interferon-tau.
MedLine Citation:
PMID:  10764976     Owner:  NLM     Status:  MEDLINE    
Objectives were to examine how the conceptus and recombinant bovine interferon-tau (rbIFN-tau) regulate intracellular components of the PGF(2a) synthetic pathway and to determine if arachidonic acid (AA) is limiting in endometrial tissue of pregnant cows. In Experiment 1, uteri were collected from either cyclic or pregnant dairy cows on Day 17 post-estrus. Intercaruncular explants were dissected and incubated for 60 min to quantify PGF(2a) production in response to oxytocin (10(-6) M), A23187 (10(-5) M), melittin (10(-5) M), and phorbol 12, 13 dibutyrate (PDBu, 10(-6) M). Additional explants from the same cows were incubated for 24 h with and without AA. Oxytocin and A23187 did not stimulate PGF(2a) in explants from either cyclic or pregnant cows. Both PDBu, melittin, and A23187 + melittin stimulated PGF(2a) production in explants of cyclic cows, but not in explants of pregnant cows. The addition of AA to explant cultures for 24 hr did not increase PGF(2a) production during a subsequent 60-min incubation. In Experiment 2, explants were collected from cows that received intrauterine infusions of either BSA (1.9 mg/1.2 ml) or rbIFN-tau (0.2 mg rbIFN-tau + 1.7 mg BSA/1.2 ml) twice a day from Days 14 to 17 of the estrous cycle. Treatments of rbIFN-tau attenuated PGF(2a) secretion induced by in vitro PDBu and A23187 treatments. However, rbIFN-tau treatment in vivo had no effect on the in vitro induction of PGF(2a) secretion by melittin. IFN-tau may regulate the PGF(2a) synthetic pathway by reducing activity of PKC or PKC mediated events.
D R Arnold; M Binelli; J Vonk; A P Alexenko; M Drost; C J Wilcox; W W Thatcher
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Domestic animal endocrinology     Volume:  18     ISSN:  0739-7240     ISO Abbreviation:  Domest. Anim. Endocrinol.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-05-23     Completed Date:  2000-05-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8505191     Medline TA:  Domest Anim Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  199-216     Citation Subset:  IM    
Department of Dairy and Poultry Sciences, University of Florida, P.O. Box 110920, Gainesville, FL, USA.
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MeSH Terms
Cattle / metabolism*
Culture Techniques
Dinoprost / biosynthesis*
Dinoprostone / biosynthesis*
Endometrium / metabolism*
Interferon Type I / pharmacology*
Pregnancy Proteins / pharmacology*
Radioimmunoassay / veterinary
Recombinant Proteins / pharmacology
Reg. No./Substance:
0/Interferon Type I; 0/Pregnancy Proteins; 0/Recombinant Proteins; 0/trophoblastin; 363-24-6/Dinoprostone; 551-11-1/Dinoprost

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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