Document Detail


Intracellular recording during magnetic field application to monitor neurotransmitter release events: methods and preliminary results.
MedLine Citation:
PMID:  2875719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for simultaneous magnetic field application and intracellular recording is presented. A little used method for magnetic field application was exploited; the field generator consisted of a flat copper sheet through which current was passed to generate a magnetic field. The resultant magnetic field was relatively homogeneous, exhibiting a variation of +/- 5%. This compact, current-sheet field generator was mounted on the condensor of a microscope. The current induced in the intracellular electrode was reduced by injecting current equal and opposite to the induced current into the microelectrode. This step reduces the possibility of cellular effects and voltage artifacts due to the induced electrode current. The technique was used to conduct preliminary studies on the effects of extremely low frequency (ELF) linearly and circularly polarized magnetic fields (1.0 Gauss, 60 and 70 Hz) on miniature end plate potential (mepp) frequency (frequency of neurotransmitter release events) of rat flexor digitorum brevis muscle. The same synapse was utilized for both the sham-exposed control and the exposed experimental halves of an experiment. After 10 min of exposure to a 60-Hz linear field, mepp frequency was significantly increased by 12%, but exposure to a 60-Hz circular field did not significantly alter mepp frequency. Exposure to a 70-Hz linear field did not significantly change mepp frequency, but application of a 70-Hz circular field appears to decrease mepp frequency by 4%. These results indicate that both types of magnetic fields can alter mepp frequency, depending upon the frequency and configuration of the field.
Authors:
R Gundersen; B Greenebaum; M Schaller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Bioelectromagnetics     Volume:  7     ISSN:  0197-8462     ISO Abbreviation:  Bioelectromagnetics     Publication Date:  1986  
Date Detail:
Created Date:  1986-10-02     Completed Date:  1986-10-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8008281     Medline TA:  Bioelectromagnetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  271-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Electromagnetic Fields*
Electromagnetic Phenomena*
Electrophysiology / methods
Membrane Potentials
Monitoring, Physiologic / methods
Motor Endplate / physiology
Neuromuscular Junction / physiology*
Neurophysiology / methods*
Neurotransmitter Agents / secretion*
Rats
Synaptic Transmission*
Grant Support
ID/Acronym/Agency:
NS 18214/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Neurotransmitter Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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