Document Detail

Intracellular pH transients in rat diaphragm muscle measured with DMO.
MedLine Citation:
PMID:  27989     Owner:  NLM     Status:  MEDLINE    
Changes of the intracellular pH of rat diaphragm muscle were monitored at 30-min intervals with the weak acid DMO (5,5-dimethyl-2,4-oxazolidinedione). Transferring the muscle from a CO2-containing to a CO2-free solution caused intracellular pH (pHi) to rise by an average of 0.18 during the first 30 min and then to level off at a slightly lower value over the next 60-90 min. Transferring the muscle from a CO2-free to a CO2-containing solution caused pHi to fall by 0.18 during the first 30 min and then to recover by 0.05 over the next 90 min. Subsequent return to the CO2-free solution caused pHi to overshoot the control value by 0.10. Both the recovery and the overshoot can be accounted for by an acid-extruding pump. Intracellular acid loading with 118 mM DMO similarly caused pHi to fall initially, to recover slowly during the acid loading, and then to overshoot the control pHi on removal of the acid load. In the absence of HCO3-/CO2, acid extrusion was reduced by about a fifth. SITS (4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid) had no effect. The absence of either Na+ or Cl- from HCO3-/CO2- free solution reduced acid extrusion by about a half.
A Roos; W F Boron
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  235     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1978 Jul 
Date Detail:
Created Date:  1978-09-29     Completed Date:  1978-09-29     Revised Date:  2009-10-27    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C49-54     Citation Subset:  IM    
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MeSH Terms
Carbon Dioxide / metabolism
Chlorides / pharmacology
Diaphragm / metabolism*
Hydrogen-Ion Concentration*
Sodium / pharmacology
Stilbenes / pharmacology
Time Factors
Reg. No./Substance:
0/Chlorides; 0/Oxazoles; 0/Stilbenes; 124-38-9/Carbon Dioxide; 695-53-4/Dimethadione; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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