| Intracellular pH regulation in the mouse lacrimal gland acinar cells. | |
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MedLine Citation:
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PMID: 2835488 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intracellular pH (pHi) of the acinar cells of the isolated, superfused mouse lacrimal gland has been measured using pH-sensitive microelectrodes. Under nonstimulated condition pHi was 7.25, which was about 0.5 unit higher than the equilibrium pH. Alterations of the external pH by +/- 0.4 unit shifted pHi only by +/- 0.08 unit. The intracellular buffering value determined by applications of 25 mM NH4+ and bicarbonate buffer solution gassed with 5% CO2/95% O2 was 26 and 46 mM/pH, respectively. Stimulation with 1 microM acetylcholine (ACh) caused a transient, small decrease and then a sustained increase in pHi. In the presence of amiloride (0.1 mM) or the absence of Na+, application of ACh caused a significant decrease in pHi and removal of amiloride or replacement with Na+-containing saline, respectively, rapidly increased the pHi. Pretreatment with DIDS (0.2 mM) did not change the pHi of the nonstimulated conditions; however, it significantly enhanced the increase in pHi induced by ACh. The present results showed that (i) there is an active acid extrusion mechanism that is stimulated by ACh; (ii) stimulation with ACh enhances the rate of acid production in the acinar cells; and (iii) the acid extrusion mechanism is inhibited by amiloride addition to and Na+ removal from the bath solution. We suggest that both Na+/H+ and HCO3-/Cl- exchange transport mechanisms are taking roles in the intracellular pH regulation in the lacrimal gland acinar cells. |
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Authors:
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Y Saito; T Ozawa; S Suzuki; A Nishiyama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of membrane biology Volume: 101 ISSN: 0022-2631 ISO Abbreviation: J. Membr. Biol. Publication Date: 1988 |
Date Detail:
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Created Date: 1988-06-14 Completed Date: 1988-06-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0211301 Medline TA: J Membr Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 73-81 Citation Subset: IM |
Affiliation:
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Department of Physiology, Tohoku University School of Medicine, Sendai, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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pharmacology Animals Carrier Proteins / metabolism Extracellular Space / physiology Homeostasis Hydrogen-Ion Concentration Intracellular Fluid / physiology Kinetics Lacrimal Apparatus / cytology, physiology* Mice Sodium-Hydrogen Antiporter |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Sodium-Hydrogen Antiporter; 51-84-3/Acetylcholine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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