| Intracellular pH in isolated rat renal papillary thin limbs of Henle's loop. | |
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MedLine Citation:
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PMID: 10864007 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intracellular pH (pHi) was measured in isolated, nonperfused and perfused rat papillary thin limbs of Henle's loops in N-2-hydroxyethylpiperazine-N'-2-ethansulfonic acid (HEPES)- or HEPES/bicarbonate-buffered medium at pH 7.4 using the pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF). Resting pHi was about 6.7 in descending thin limbs (DTL) and about 6.9 in ascending thin limbs (ATL), even with a medium pH of 7.4. These values appeared to reflect the acid pH of the blood in the neighboring vasa recta found in vivo. The resting pHi did not differ whether or not the medium contained bicarbonate although the total buffering capacity of the tubule cells was increased in the presence of bicarbonate. In nonperfused DTL and ATL, pHi was further acidified following an NH4Cl pulse. The rate of recovery of pHi from this level to the resting pHi was reduced by Na+ removal from the bath in both DTL and ATL and by the addition of ethylisopropylamiloride (EIPA) to the bath in the presence of Na+ in DTL. The rate of recovery was not affected by Cl- removal from the bath or K+ (75 mM) or 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS) addition to the bath in either DTL or ATL. These results suggest that the common, amiloride-sensitive, basolateral Na+/H+ exchanger plays a role in the regulation of pHi in rat papillary DTL but that a different basolateral Na+/H+ exchanger or a luminal Na+/H+ exchanger is important in rat papillary ATL. |
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Authors:
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W H Dantzler; Y K Kim; D E Abbott; O K Serrano; O H Brokl |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 440 ISSN: 0031-6768 ISO Abbreviation: Pflugers Arch. Publication Date: 2000 May |
Date Detail:
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Created Date: 2000-08-16 Completed Date: 2000-08-16 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: GERMANY |
Other Details:
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Languages: eng Pagination: 140-8 Citation Subset: IM |
Affiliation:
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Department of Physiology, College of Medicine, University of Arizona, Tucson 85724-5051, USA. dantzler@u.arizona.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
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pharmacology Acid-Base Equilibrium / physiology* Amiloride / analogs & derivatives*, pharmacology Ammonia / metabolism Ammonium Chloride / pharmacology Animals Bicarbonates / metabolism, pharmacology Carrier Proteins / antagonists & inhibitors, metabolism Cell Membrane Permeability / drug effects Chlorides / metabolism Fluoresceins Fluorescent Dyes Intracellular Fluid / metabolism* Loop of Henle / cytology, metabolism* Male Perfusion Potassium / metabolism, pharmacology Rats Rats, Wistar Sodium / metabolism Sodium-Bicarbonate Symporters Sodium-Hydrogen Antiporter / drug effects, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DK 16294/DK/NIDDK NIH HHS; HL-07249/HL/NHLBI NIH HHS; NS-07309/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bicarbonates; 0/Carrier Proteins; 0/Chlorides; 0/Fluoresceins; 0/Fluorescent Dyes; 0/Sodium-Bicarbonate Symporters; 0/Sodium-Hydrogen Antiporter; 1154-25-2/ethylisopropylamiloride; 12125-02-9/Ammonium Chloride; 2609-46-3/Amiloride; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 7440-09-7/Potassium; 7440-23-5/Sodium; 7664-41-7/Ammonia; 85138-49-4/2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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