Document Detail


Intracellular pH homeostasis of Candida glabrata in infection associated conditions.
MedLine Citation:
PMID:  23378571     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Candida glabrata is an opportunistic fungal pathogen which is a growing concern for immunocompromised patients. It is ranked as second most common cause of candidiasis after Candida albicans. For pathogenic yeasts, intracellular pH (pHi) has been implicated in proliferation, dimorphic switching and virulence. We expressed the pH-sensitive green fluorescent protein (GFP) variant ratiometric pHluorin in the cytosol of C. glabrata to study pHi dynamics in living cells. We evaluated the response of pHi to the various growth and stress conditions encountered during interaction with the host and during antifungal treatment. C. glabrata maintained a pHi higher than that of Saccharomyces cerevisiae in all growth conditions. The pHi of S. cerevisiae cells appeared better controlled than the pHi in C. glabrata when the cells were exposed to food and fermentation associated conditions. C. glabrata in turn maintained its pHi better when exposed to host associated conditions.
Authors:
Azmat Ullah; Maria Ines Lopes; Stanley Brul; Gertien J Smits
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-1
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  -     ISSN:  1465-2080     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Swammerdam Institute for Life Sciences, University of Amsterdam.
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