Document Detail


Intracellular pH change does not appear as a prerequisite for triggering activation of Barnea candida (Mollusca, Pelecypoda) oocytes.
MedLine Citation:
PMID:  3235027     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Barnea candida oocytes, exposed to excess KCl, ammonia, or digitonin, exhibit germinal vesicle breakdown (GVBD) and reinitiate meiosis, at least up to first polar body extrusion. While we confirm that KCl- but not ammonia-induced activation required external calcium, our findings that digitonin is effective at any pH from 6 to 8, in the presence of calcium, while the phorbol ester TPA and diacylglycerol fail to reinitiate meiosis, strongly suggests calcium as the main trigger for this process. Preliminary experiments using the fluorescent probes fluorescein diacetate and Quin 2/AM show, moreover, that KCl and ammonia produce both an intracellular calcium surge (30 nM) and a slight alkalinization of the intracellular cytoplasm from 7.84 to 8.05.
Authors:
M Brassard; H Duclohier; M Moreau; P Guerrier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gamete research     Volume:  20     ISSN:  0148-7280     ISO Abbreviation:  Gamete Res     Publication Date:  1988 May 
Date Detail:
Created Date:  1989-04-27     Completed Date:  1989-04-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7806559     Medline TA:  Gamete Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  43-52     Citation Subset:  IM    
Affiliation:
Developmental Biology, CNRS, Roscoff, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bivalvia / physiology*
Female
Hydrogen-Ion Concentration
Male
Oocytes / physiology*
Sperm-Ovum Interactions*

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