| Intracellular oxidative activity and respiratory burst of leukocytes isolated from multiple sclerosis patients. | |
| | |
MedLine Citation:
|
PMID: 16263194 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Oxidative damage induced by free radicals and reactive oxygen species (ROS) have been suggested to play an important role in the development of autoimmune diseases such as multiple sclerosis (MS) disease and it has been hypothesised that oxidative injury could mediate demyelination and axonal injury in MS subjects. In our study, we compared intracellular oxidative activity and the respiratory burst activity in MS patients (n=20) and healthy controls (n=15) using leukocytes as cellular model. At this purpose, intracellular ROS levels were evaluated by fluorometric assay using the 2'-7'-dichlorodihydrofluorescin diacetate probe (H(2)DCFDA) in untreated or in leukocytes stimulated with phorbol-12-myristate-13-acetate (PMA). Our results demonstrate that the intracellular spontaneous ROS production in leukocytes from MS patients was higher with respect to cells from control subjects (p<0.001). PMA addition induced a higher formation of ROS both in leukocytes from MS patients and controls (p<0.001). The PMA-induced production of ROS was significantly higher in leukocytes from MS with respect to controls (p<0.001). Significant positive correlations were established between intracellular spontaneous or PMA-induced production of ROS in leukocytes isolated from MS patients and the clinical parameters used to evaluate disease disability such as expanded disability status scale (EDSS), brain lesions evaluated by MRI and visual evoked potential (VEP) (p<0.001). In conclusion, our results demonstrate higher levels of intracellular ROS in untreated or in PMA-treated leukocytes isolated from MS patients with respect to healthy subjects confirming the role of oxidative stress in multiple sclerosis. |
| | |
Authors:
|
G Ferretti; T Bacchetti; F DiLudovico; B Viti; V A Angeleri; M Danni; L Provinciali |
Related Documents
:
|
19752304 - Multiple sclerosis with predominant, severe cognitive impairment. 11254774 - Demyelinating disorder of the central nervous system occurring in black south africans. 8289084 - Cardiovascular autonomic dysfunction in multiple sclerosis is likely related to brainst... 20976784 - Reproducibility of the motor unit number index (munix) in normal controls and amyotroph... 331874 - Synaptic membrane protein d2 in the cerebrospinal fluid of manic-melancholic patients. 3656464 - Hypothermia in trauma victims: an ominous predictor of survival. |
Publication Detail:
|
Type: Journal Article Date: 2005-11-02 |
Journal Detail:
|
Title: Neurochemistry international Volume: 48 ISSN: 0197-0186 ISO Abbreviation: Neurochem. Int. Publication Date: 2006 Jan |
Date Detail:
|
Created Date: 2005-12-30 Completed Date: 2006-04-24 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8006959 Medline TA: Neurochem Int Country: England |
Other Details:
|
Languages: eng Pagination: 87-92 Citation Subset: IM |
Affiliation:
|
Istituto di Biochimica, Facoltà di Medicina e Chirurgia, Università Politecnica delle Marche, Via Ranieri, 60131 Ancona, Italy. g.ferretti@univpm.it |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Case-Control Studies Evoked Potentials, Visual Female Fluoresceins / chemistry Fluorescent Dyes / chemistry Humans Leukocytes / drug effects, metabolism* Magnetic Resonance Imaging Male Middle Aged Multiple Sclerosis / blood*, metabolism, physiopathology Oxidative Stress* Respiratory Burst Tetradecanoylphorbol Acetate / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Fluoresceins; 0/Fluorescent Dyes; 106070-31-9/2',7'-dichlorodihydrofluorescein; 16561-29-8/Tetradecanoylphorbol Acetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intraocular sustained drug delivery using implantable polymeric devices.
Next Document: Synthesis of new hexahydro- and octahydropyrido[1,2-c]pyrimidine derivatives with an arylpiperazine ...