Document Detail

Intracellular oxidative activity and respiratory burst of leukocytes isolated from multiple sclerosis patients.
MedLine Citation:
PMID:  16263194     Owner:  NLM     Status:  MEDLINE    
Oxidative damage induced by free radicals and reactive oxygen species (ROS) have been suggested to play an important role in the development of autoimmune diseases such as multiple sclerosis (MS) disease and it has been hypothesised that oxidative injury could mediate demyelination and axonal injury in MS subjects. In our study, we compared intracellular oxidative activity and the respiratory burst activity in MS patients (n=20) and healthy controls (n=15) using leukocytes as cellular model. At this purpose, intracellular ROS levels were evaluated by fluorometric assay using the 2'-7'-dichlorodihydrofluorescin diacetate probe (H(2)DCFDA) in untreated or in leukocytes stimulated with phorbol-12-myristate-13-acetate (PMA). Our results demonstrate that the intracellular spontaneous ROS production in leukocytes from MS patients was higher with respect to cells from control subjects (p<0.001). PMA addition induced a higher formation of ROS both in leukocytes from MS patients and controls (p<0.001). The PMA-induced production of ROS was significantly higher in leukocytes from MS with respect to controls (p<0.001). Significant positive correlations were established between intracellular spontaneous or PMA-induced production of ROS in leukocytes isolated from MS patients and the clinical parameters used to evaluate disease disability such as expanded disability status scale (EDSS), brain lesions evaluated by MRI and visual evoked potential (VEP) (p<0.001). In conclusion, our results demonstrate higher levels of intracellular ROS in untreated or in PMA-treated leukocytes isolated from MS patients with respect to healthy subjects confirming the role of oxidative stress in multiple sclerosis.
G Ferretti; T Bacchetti; F DiLudovico; B Viti; V A Angeleri; M Danni; L Provinciali
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Publication Detail:
Type:  Journal Article     Date:  2005-11-02
Journal Detail:
Title:  Neurochemistry international     Volume:  48     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-30     Completed Date:  2006-04-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  87-92     Citation Subset:  IM    
Istituto di Biochimica, Facoltà di Medicina e Chirurgia, Università Politecnica delle Marche, Via Ranieri, 60131 Ancona, Italy.
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MeSH Terms
Case-Control Studies
Evoked Potentials, Visual
Fluoresceins / chemistry
Fluorescent Dyes / chemistry
Leukocytes / drug effects,  metabolism*
Magnetic Resonance Imaging
Middle Aged
Multiple Sclerosis / blood*,  metabolism,  physiopathology
Oxidative Stress*
Respiratory Burst
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
0/Fluoresceins; 0/Fluorescent Dyes; 106070-31-9/2',7'-dichlorodihydrofluorescein; 16561-29-8/Tetradecanoylphorbol Acetate

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