Document Detail

Intracellular melatonin distribution in cultured cell lines.
MedLine Citation:
PMID:  9468115     Owner:  NLM     Status:  MEDLINE    
A specific antibody combined with a fluorescein-labeled immunoglobulin was used to investigate the topographic distribution of melatonin in a variety of cells of different origins. Positive identification of both nuclear and cytosolic melatonin was confirmed in all the tested cells: Swiss 3T3 mouse fibroblasts, BCG1 bovine granulosa, NB41A3 mouse neuroblastoma, F9 mouse teratocarcinoma, MDCK normal canine kidney derived and human HeLa cell lines, as well as in human peripheral blood mononuclear leukocytes and rat splenic cells. In 3T3 mouse fibroblasts melatonin immunofluorescence partially colocalized with actin and serotonin immunostaining, but not with tubulin or actin stress fibers. Several distinct patterns of subcellular melatonin distribution, different from the bromodeoxyuridine-labeled replication profiles, have been discerned throughout the cell cycle of synchronized 3T3 cells. In addition, synchronized 3T3 mouse fibroblasts cultured in the presence of 10(-3) M melatonin progressed more slowly through the cell cycle than control cells. These results suggest that melatonin may interact directly with nuclear and cytoskeletal structures probably affecting different cell functions such as cell cycle control, subcellular organization, and genome stability.
L M Finocchiaro; G C Glikin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pineal research     Volume:  24     ISSN:  0742-3098     ISO Abbreviation:  J. Pineal Res.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-02-26     Completed Date:  1998-02-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8504412     Medline TA:  J Pineal Res     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  22-34     Citation Subset:  IM    
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
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MeSH Terms
Bromodeoxyuridine / metabolism
Cell Culture Techniques
Cell Cycle
Cell Line / metabolism
Cell Nucleus / metabolism
Cytoskeletal Proteins / metabolism
Cytosol / metabolism
DNA / biosynthesis
DNA Replication
Fluorescent Antibody Technique, Indirect
Melatonin / metabolism*
Subcellular Fractions / metabolism
Reg. No./Substance:
0/Cytoskeletal Proteins; 59-14-3/Bromodeoxyuridine; 73-31-4/Melatonin; 9007-49-2/DNA

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