Document Detail

Intracellular localization of human ZBP1: Differential regulation by the Z-DNA binding domain, Zalpha, in splice variants.
MedLine Citation:
PMID:  16876127     Owner:  NLM     Status:  MEDLINE    
We investigated the subcellular distribution of human ZBP1, which harbors the N-terminal Z-DNA binding domains, Zalpha and Zbeta. ZBP1 was distributed primarily in the cytoplasm and occasionally as nuclear foci in interferon (IFN)-treated primary hepatocellular carcinoma cells, and in several other transfected cell types. In leptomycin B (LMB)-treated cells, endogenous ZBP1 efficiently accumulated in nuclear foci, which overlapped PML oncogenic domains (PODs) or nuclear bodies (NBs). In transfection assays, the unique C-terminal region of ZBP1 was necessary for its typical cytoplasmic localization. Interestingly, the Zalpha-deleted form displayed an increased association with PODs compared to wild-type and, unlike wild-type, perfectly accumulated in PODs in LMB-treated cells, implying that the presence of Zalpha domain also facilitates the cytoplasmic localization. Our results demonstrate that ZBP1 is localized primarily in the cytoplasm but also associated with nuclear PODs in IFN or LMB-treated cells. Given that about half of ZBP1 mRNA lacks exon 2 encoding the Zalpha domain, our data also suggest that the localization of ZBP1 may be differentially regulated by the Z-DNA binding domain, Zalpha, in splice variants.
Hong Thanh Pham; Mi-Young Park; Kyeong Kyu Kim; Yang-Gyun Kim; Jin-Hyun Ahn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-24
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  348     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-11     Completed Date:  2006-11-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  145-52     Citation Subset:  IM    
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Sungkyunkwan University, Suwon, Republic of Korea.
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MeSH Terms
Antibiotics, Antineoplastic / pharmacology
Binding Sites
Cell Line, Tumor
Cell Nucleus / metabolism
Cercopithecus aethiops
Cytoplasm / metabolism
DNA, Z-Form / metabolism*
DNA-Binding Proteins / metabolism
Fatty Acids, Unsaturated / pharmacology
Glycoproteins / analysis,  chemistry,  genetics,  metabolism*
Interferon-gamma / pharmacology
Models, Molecular
Protein Binding
Protein Structure, Tertiary
RNA Splicing
Vero Cells
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/DNA, Z-Form; 0/DNA-Binding Proteins; 0/Fatty Acids, Unsaturated; 0/Glycoproteins; 0/ZBP1 protein, human; 82115-62-6/Interferon-gamma; 87081-35-4/leptomycin B

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