| Intracellular localization of drugs in cultured tumor cells by ion microscopy and image processing. | |
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MedLine Citation:
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PMID: 2139017 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several drugs, containing a halogen atom, F or Br, that are being used in antiviral or anticancer therapy, were studied for their localization in cultured cells by ion microanalysis. The association allows to reduce the exposure time to define the intracellular localization of the studied element. The topography of the cells is given by the image of the polyatomic ion 26CN-. The image of the distribution of 81Br- or 19F-, coded in another color scale, can be superimposed, giving a polychromic image of the cell, thus showing the intracellular localization of the compound. MCF-7 tumor cells were cultured in the presence of pyrimidine derivatives. 5-Bromo-2'-deoxyuridine (BUdR) and 5-trifluorothymidine (F3TdR) were localized in the nucleus, 5-fluoro-2'-deoxyuridine (FUdR) in the nucleus and only in some nucleoli. The method is simple and rapid, as compared with techniques using radiolabeled compounds, or with immunocytochemical techniques. It is possible to observe two different compounds in the same cell. It could be applied to other compounds containing a halogen atom. |
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Authors:
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J P Berry; G Lespinats; F Escaig; P Boumati; S Tlouzeau; J F Cavellier |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Histochemistry Volume: 93 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1990 |
Date Detail:
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Created Date: 1990-05-24 Completed Date: 1990-05-24 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 397-400 Citation Subset: IM |
Affiliation:
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SC 27 de l'INSERM, Faculté de Médicine, Créteil, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bromodeoxyuridine
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analysis* Cyanides / analysis Floxuridine / analysis* Halogens / analysis Humans Image Processing, Computer-Assisted / methods Ions Microscopy / methods Thymidine / analogs & derivatives* Trifluridine / analysis* Tumor Cells, Cultured / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Cyanides; 0/Halogens; 0/Ions; 50-89-5/Thymidine; 50-91-9/Floxuridine; 59-14-3/Bromodeoxyuridine; 70-00-8/Trifluridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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