Document Detail


Intracellular localization of PNA in human cells upon its introduction by electroporation.
MedLine Citation:
PMID:  22545409     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Peptide nucleic acid (PNA) is one of the most useful DNA analogs in a wide variety of gene analysis in human cells. In order to exhibit its maximal functions, PNA must be localized to a desired place (e.g., nucleus, cytoplasm and other organelles). Here, we introduced PNAs into HeLa cells by electroporation and examined their localization at various time points. The PNA which binds to the mitochondrial COII gene was initially accumulated in the nucleus, and thereafter mostly transferred to cytoplasm. This time-dependent intracellular localization of PNA is ascribed to the breakdown of the nuclear envelope in the cell division. On the other hand, another PNA that binds to telomere repeat sequence mostly remained in the nucleus, even after the cell division occurred. The retention of this PNA in the nucleus was further enhanced when it was conjugated with Cy3.
Authors:
Eri Noguchi; Narumi Shigi; Makoto Komiyama
Related Documents :
14720299 - Use of viral promoters in mammalian cell-based bioassays: how reliable?
19739429 - Inhibition effects of vitex rotundifolia on inflammatory gene expression in a549 human ...
8804409 - Pwp2, a member of the wd-repeat family of proteins, is an essential saccharomyces cerev...
19286049 - Food flavonoid aryl hydrocarbon receptor-mediated agonistic/antagonistic/synergic activ...
8316249 - Identification of the activation-labile gene: a single point mutation in the human gluc...
18256039 - Regulation of the plasma cell transcription factor blimp-1 gene by bach2 and bcl6.
9316779 - Usefulness of computer-aided karyometric examination in preoperative differentiation of...
20550519 - Resistance to experimental tumorigenesis in cells of a long-lived mammal, the naked mol...
20883819 - The lens epithelium in ocular health and disease.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Natural product communications     Volume:  7     ISSN:  1934-578X     ISO Abbreviation:  Nat Prod Commun     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-05-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101477873     Medline TA:  Nat Prod Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  349-52     Citation Subset:  IM    
Affiliation:
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, Japan 153-8904.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Monitoring stepwise proteolytic degradation of peptides by supramolecular domino tandem assays and m...
Next Document:  Fliposomes: pH-controlled release from liposomes containing new trans-2-morpholinocyclohexanol-based...