Document Detail


Intracellular localization of DNA polymerase alpha.
MedLine Citation:
PMID:  7019918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immunoglobulin (IgG) and the F(ab')2 fragment of IgG were prepared from serum of a rabbit immunized with purified calf thymus DNA polymerase alpha (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7). An indirect immunofluorescent method based on these reagents was used to detect the intracellular localization of DNA polymerase alpha in primary fetal bovine fibroblasts. The results show that the bulk of DNA polymerase alpha is located in the perinuclear region of the cytoplasm. Immunofluorescent staining of cytoplast and Ficoll-Paque gradient-purified karyoplast fragments resulting from cytochalasin enucleation show the presence of DNA polymerase alpha in cytoplasts and the virtual absence of the enzyme in the nucleus of the karyoplast itself. The implication of this unusual intracellular location for DNA polymerase alpha is discussed.
Authors:
M Brown; F J Bollum; L M Chang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  78     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1981 May 
Date Detail:
Created Date:  1981-09-25     Completed Date:  1981-09-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3049-52     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cells, Cultured
Cytochalasin B / pharmacology
DNA Polymerase II / analysis*
DNA-Directed DNA Polymerase / analysis*
Female
Fetus
Fibroblasts / enzymology
Fluorescent Antibody Technique
Immunoglobulin Fab Fragments
Immunoglobulin G
Kidney / drug effects,  enzymology*
Pregnancy
Grant Support
ID/Acronym/Agency:
CA 23262/CA/NCI NIH HHS; CA 23365/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Immunoglobulin Fab Fragments; 0/Immunoglobulin G; 14930-96-2/Cytochalasin B; EC 2.7.7.-/DNA Polymerase II; EC 2.7.7.7/DNA-Directed DNA Polymerase
Comments/Corrections

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