Document Detail

Intracellular labeling of single cortical astrocytes in vivo.
MedLine Citation:
PMID:  17686526     Owner:  NLM     Status:  MEDLINE    
Glial cells have traditionally been considered to play supportive roles in the central nervous system. As recent experimental evidence suggests glial cells' participation in neural information processing, there has been a need to monitor the physiology of glial cells in vivo in the matured brain. Concurrently, identification and classification of the recorded glial cells is essential as there are at least several different kinds of glial cells. Past studies have achieved in vivo intracellular electrophysiological recording of glial cells using sharp glass microelectrodes, however, morphological recovery and identification of the recorded cells have hardly been done, due to technical difficulties. We demonstrate that use of large fragment biotinylated dextran amine (BDA) is an effective way to label a single glial cell recorded with a sharp microelectrode in vivo. Furthermore, the tracer signal amplification was achieved by a combination of avidin biotinylated horseradish peroxidase macromolecular complex (ABC) and tyramide-based methods, making multiple immunohistochemistry feasible. Using the method described in this study, we have successfully recorded and labeled cortical glial cells including astrocytes, oligodendrocytes, and microglia.
Tsuneko Mishima; Seiichi Sakatani; Hajime Hirase
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-01
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  166     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-17     Completed Date:  2007-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  32-40     Citation Subset:  IM    
Hirase Research Unit, Neuronal Circuit Mechanisms Research Group, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
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MeSH Terms
Astrocytes / cytology*,  metabolism
Bacterial Proteins / chemistry
Biological Markers / metabolism
Biotin / analogs & derivatives*,  chemistry
Cell Shape / physiology
Cerebral Cortex / cytology*,  metabolism
Electrophysiology / instrumentation,  methods*
Glial Fibrillary Acidic Protein / metabolism
Horseradish Peroxidase / chemistry
Immunohistochemistry / methods*
Intracellular Fluid / chemistry,  metabolism
Microelectrodes / standards
Microglia / cytology,  metabolism
Oligodendroglia / cytology,  metabolism
Rats, Sprague-Dawley
Staining and Labeling / instrumentation,  methods*
Tyramine / chemistry
Reg. No./Substance:
0/Bacterial Proteins; 0/Biological Markers; 0/Glial Fibrillary Acidic Protein; 0/Strep-avidin conjugated horseradish peroxidase; 0/biotinylated dextran amine; 51-67-2/Tyramine; 58-85-5/Biotin; 9004-54-0/Dextrans; EC 1.11.1.-/Horseradish Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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