| Intracellular labeling of single cortical astrocytes in vivo. | |
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MedLine Citation:
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PMID: 17686526 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glial cells have traditionally been considered to play supportive roles in the central nervous system. As recent experimental evidence suggests glial cells' participation in neural information processing, there has been a need to monitor the physiology of glial cells in vivo in the matured brain. Concurrently, identification and classification of the recorded glial cells is essential as there are at least several different kinds of glial cells. Past studies have achieved in vivo intracellular electrophysiological recording of glial cells using sharp glass microelectrodes, however, morphological recovery and identification of the recorded cells have hardly been done, due to technical difficulties. We demonstrate that use of large fragment biotinylated dextran amine (BDA) is an effective way to label a single glial cell recorded with a sharp microelectrode in vivo. Furthermore, the tracer signal amplification was achieved by a combination of avidin biotinylated horseradish peroxidase macromolecular complex (ABC) and tyramide-based methods, making multiple immunohistochemistry feasible. Using the method described in this study, we have successfully recorded and labeled cortical glial cells including astrocytes, oligodendrocytes, and microglia. |
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Authors:
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Tsuneko Mishima; Seiichi Sakatani; Hajime Hirase |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-07-01 |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 166 ISSN: 0165-0270 ISO Abbreviation: J. Neurosci. Methods Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-09-17 Completed Date: 2007-12-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 32-40 Citation Subset: IM |
Affiliation:
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Hirase Research Unit, Neuronal Circuit Mechanisms Research Group, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astrocytes / cytology*, metabolism Bacterial Proteins / chemistry Biological Markers / metabolism Biotin / analogs & derivatives*, chemistry Cell Shape / physiology Cerebral Cortex / cytology*, metabolism Dextrans* Electrophysiology / instrumentation, methods* Glial Fibrillary Acidic Protein / metabolism Horseradish Peroxidase / chemistry Immunohistochemistry / methods* Intracellular Fluid / chemistry, metabolism Microelectrodes / standards Microglia / cytology, metabolism Oligodendroglia / cytology, metabolism Rats Rats, Sprague-Dawley Staining and Labeling / instrumentation, methods* Tyramine / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Biological Markers; 0/Glial Fibrillary Acidic Protein; 0/Strep-avidin conjugated horseradish peroxidase; 0/biotinylated dextran amine; 51-67-2/Tyramine; 58-85-5/Biotin; 9004-54-0/Dextrans; EC 1.11.1.-/Horseradish Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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