| Intracellular filament bundles in whole mounts of chick and human myoblasts extracted with triton X-100. | |
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MedLine Citation:
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PMID: 7193921 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The method of Triton X-100 extraction and critical point drying of whole mounts of cultured chick and human myoblasts was used to study the presence of intracellular bundles of filaments within these cells. Observation by means of transmission and scanning electron microscopy demonstrated a complex system of filament bundles which appeared morphologically and spatially heterogeneous. Most obvious were long dense bundles of cables traversing along the ventral surface of developing myoblasts, presumably the 'stress fibers' seen in light microscopy. Other bundle types occurred which were composed of loose aggregates of filaments coursing through the remnant cell body. A prominent accumulation of filaments was also seen at the lateral edges of these myoblasts. These lateral edge cables were thicker and denser than any other type of filament bundle observed in the myoblasts. Reaction of unextracted myoblasts directly to human antiplatelet myosin conjugated to rhodamine demonstrated that the most intense reaction also occurred along the lateral edges of both human and chick myoblasts. During development of chick myoblasts the filament bundles became oriented parallel to the cell axis giving the cell a fusiform morphology. It is possible that the various filament bundle structures and their differing structural and spatial dispositions could be related to functional differences among the diverse population of intracellular bundles of filaments. |
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Authors:
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J Pudney; R H Singer |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Tissue & cell Volume: 12 ISSN: 0040-8166 ISO Abbreviation: Tissue Cell Publication Date: 1980 |
Date Detail:
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Created Date: 1981-05-13 Completed Date: 1981-05-13 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0214745 Medline TA: Tissue Cell Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 595-612 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation Cells, Cultured Chick Embryo Cytoskeleton / analysis, ultrastructure* Humans Microscopy, Electron Muscles / analysis, ultrastructure* Myosins / analysis Polyethylene Glycols |
| Grant Support | |
ID/Acronym/Agency:
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NS 11329/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Polyethylene Glycols; EC 3.6.4.1/Myosins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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