Document Detail

Intracellular events in retinal glial cells exposed to ICG and BBG.
MedLine Citation:
PMID:  17898261     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To investigate the intracellular events in retinal glial cells exposed to indocyanine green (ICG) and brilliant blue G (BBG). METHODS: The human Müller cell line MIO-M1 was exposed to a low dose (0.25 mg/mL) and a clinical dose (2.5 mg/mL) of ICG and a clinical dose (0.25 mg/mL) of BBG for 15 minutes, respectively. To quantify the proliferation and viability of the cells, [(3)H]-thymidine incorporation was measured and cell numbers were counted 24 hours after treatment. Cell morphology was evaluated using phase-contrast microscopy and transmission electron microscopy. The effects of ICG and BBG on phosphorylation of p38 MAPK and cleavage of caspase-9 and caspase-3 were examined by Western blot. RESULTS: ICG and BBG significantly reduced [(3)H]-thymidine incorporation in MIO-M1 cells compared with the vehicle-treated controls (P < 0.01). Cell number significantly decreased after exposure to ICG at 2.5 or 0.25 mg/mL (P < 0.01) but did not decrease after exposure to BBG at 0.25 mg/mL. Transmission electron microscopy revealed apoptotic changes only in the ICG-treated cells. Prominent p38 MAPK phosphorylation was observed in the presence of ICG, even at the low concentration and within a short time exposure; however, no apparent enhancement was observed in the presence of 0.25 mg/mL BBG. Furthermore, ICG, but not BBG, induced the cleavage of caspase-9 and caspase-3, which was inhibited by an inhibitor of p38 MAPK. CONCLUSIONS: ICG is toxic to retinal glial cells because it induces apoptosis, involving induction of the caspase cascade through p38 MAPK phosphorylation. In contrast, BBG does not cause apoptosis and thus could be a safer adjuvant during vitreoretinal surgery.
Shuhei Kawahara; Yasuaki Hata; Muneki Miura; Takeshi Kita; Akihito Sengoku; Shintaro Nakao; Yasutaka Mochizuki; Hiroshi Enaida; Akifumi Ueno; Ali Hafezi-Moghadam; Tatsuro Ishibashi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  48     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-27     Completed Date:  2007-11-01     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4426-32     Citation Subset:  IM    
Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
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MeSH Terms
Apoptosis / drug effects
Blotting, Western
Caspase 3 / metabolism
Caspase 9 / metabolism
Cell Count
Cell Culture Techniques
Cell Proliferation / drug effects
Cell Survival / drug effects
Enzyme Activation
Indocyanine Green / toxicity*
Microscopy, Electron, Transmission
Microscopy, Phase-Contrast
Neuroglia / drug effects*,  metabolism,  pathology
Retina / drug effects*,  metabolism,  pathology
Rosaniline Dyes / toxicity*
p38 Mitogen-Activated Protein Kinases / metabolism
Reg. No./Substance:
0/Rosaniline Dyes; 3599-32-4/Indocyanine Green; 6104-58-1/coomassie Brilliant Blue; EC Mitogen-Activated Protein Kinases; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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