| Intracellular distribution of tamoxifen in resistant human breast adenocarcinoma cells using tamoxifen-eosin association. | |
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MedLine Citation:
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PMID: 9879935 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A tamoxifen-resistant cell line (MCF7TAM) was established from tamoxifen-sensitive MCF-7 human breast cancer cells expressing estrogen receptors. Though the resistant cell line grows in the presence of tamoxifen, estrogen receptors continue to be expressed at similar levels as in the parental cell line. However, estrogen receptors appeared to be altered in the resistant cell line since important discrepancies are observed between results obtained with ligand binding assays and immunoenzymatic assays, tending to show modifications of estrogen receptor ligand binding capacity. The intracellular distribution of tamoxifen in sensitive and resistant cell lines was investigated using fluorescence of eosin-tamoxifen ionic association. Fluorescence emission spectra of eosin, tamoxifen and eosin-tamoxifen complex (lambda(ex)=480 nm) were analyzed and the maximal fluorescence intensity found for the complex (lambda(em)=540 nm) was four times higher than that of eosin alone, while tamoxifen alone did not emit any fluorescence in this spectral range. In MCF-7 cells, tamoxifen was found to be mainly located surrounding the nucleus, although nuclear fluorescence intensity was significantly lower. No highly fluorescent granules were observed in the resistant cell lines as opposed to sensitive cells. Improvement of this fluorescence microscopy methodology could appear of interest, taking into account the complexity of tamoxifen resistance molecular pathways. |
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Authors:
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N Bachmann; M Barberi-Heyob; C Bour; R M Parache; F Guillemin; A M Batt; J L Merlin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biology and toxicology Volume: 14 ISSN: 0742-2091 ISO Abbreviation: Cell Biol. Toxicol. Publication Date: 1998 Dec |
Date Detail:
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Created Date: 1999-03-11 Completed Date: 1999-03-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8506639 Medline TA: Cell Biol Toxicol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 429-35 Citation Subset: IM |
Affiliation:
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Laboratoire de Recherche en Oncologie, Centre Alexis Vautrin, Vandoeuvre-les-Nancy, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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metabolism*,
pathology Antineoplastic Agents, Phytogenic / chemistry, metabolism* Breast Neoplasms / metabolism*, pathology Drug Resistance, Neoplasm Eosine Yellowish-(YS) / chemistry* Humans Microscopy, Fluorescence Spectrometry, Fluorescence Tamoxifen / chemistry, metabolism* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 10540-29-1/Tamoxifen; 17372-87-1/Eosine Yellowish-(YS) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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