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Intracellular distribution of alkaline phosphatase in rat liver cells.
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MedLine Citation:
PMID:  13242596     Owner:  NLM     Status:  MEDLINE    
1. Cytochemical studies of the intracellular distribution of alkaline phosphatase in rat liver have been made, using a fractionation procedure recently developed in this laboratory (8) and a similar but modified method not described previously. Aqueous media were used in both cases. 2. The alkaline phosphatase was found to consist of two forms, one of which is strongly activated by magnesium and one of which is not sensitive to this metal. 3. The form of the enzyme that is not activated by magnesium occurs mainly in the nuclear fraction, where it seems to be rather firmly bound. Some of this form of the enzyme is also found in the microsomes, but very little if any occurs in the soluble supernatant fraction. 4. The form of alkaline phosphatase which is activated by magnesium occurs mainly in the soluble supernatant fraction, but what is believed are significant amounts also occur in nuclei. A significant portion of this form of the enzyme can be extracted from the isolated nuclei with cold, isotonic saline solution. Some activity of this form of the enzyme is also found in the microsomal fraction. 5. Mitochondria appear to contain relatively little alkaline phosphatase of either kind. 6. The concept of a porous nuclear membrane has been invoked to explain some of the results obtained in this work. It is postulated that part at least of the form of the enzyme that is activated by magnesium is free to diffuse back and forth through pores in the nuclear membrane, whereas this is considered not to be possible for the form of the enzyme that is insensitive to magnesium as a result of the firm binding of the latter to nuclear substance.
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biophysical and biochemical cytology     Volume:  1     ISSN:  0095-9901     ISO Abbreviation:  J Biophys Biochem Cytol     Publication Date:  1955 Jul 
Date Detail:
Created Date:  1955-12-01     Completed Date:  2003-05-01     Revised Date:  2010-09-21    
Medline Journal Info:
Nlm Unique ID:  17840020R     Medline TA:  J Biophys Biochem Cytol     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  315-30     Citation Subset:  OM    
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MeSH Terms
Liver / metabolism*
Phosphoric Monoester Hydrolases / analysis*
Reg. No./Substance:
EC 3.1.3.-/Phosphoric Monoester Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Biophys Biochem Cytol
ISSN: 0095-9901
Publisher: The Rockefeller University Press
Article Information
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Copyright © Copyright, 1955, by The Rockefeller Institute for Medical Research
Received Day: 14 Month: 4 Year: 1955
Print publication date: Day: 25 Month: 7 Year: 1955
Volume: 1 Issue: 4
First Page: 315 Last Page: 330
ID: 2223818
PubMed Id: 13242596

Arthur J. Emery, Jr.
Alexander L. Dounce
(From the Department of Biochemistry, The University of Rochester School of Medicine and Dentistry, Rochester, New York)

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