Document Detail


Intracellular diffusion of oxygen and hypoxic sensing: role of mitochondrial respiration.
MedLine Citation:
PMID:  20217352     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In vivo, diffusional O(2) gradients from the capillary blood to the intracellular space determine O(2) availability at the O(2) sensing molecules in the cell. With a novel technique for imaging intracellular O(2) levels using green fluorescent protein (GFP), we examined the possibility that diffusional O(2) concentration gradients might be involved in the cellular hypoxic sensing in cultured Hep3B cells. In the present study, we failed to demonstrate significant gradients of intracellular O(2) when mitochondrial respiration was maximally elevated by an uncoupler of oxidative phosphorylation. Thus, we conclude that intracellular O(2) gradients may be negligible at normal mitochondrial O(2) demand in these cells.
Authors:
Eiji Takahashi; Michihiko Sato
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  669     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-03-10     Completed Date:  2010-05-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  213-7     Citation Subset:  IM    
Affiliation:
Department of Physiology, Yamagata University School of Medicine, Yamagata, Japan. eiji@med.id.yamagata-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Anoxia / metabolism*
Cell Line, Tumor
Cell Respiration
Diffusion
Humans
Intracellular Space / metabolism*
Mitochondria / metabolism*
Oxygen / metabolism*
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
7782-44-7/Oxygen

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