| Intracellular diffusion of oxygen and hypoxic sensing: role of mitochondrial respiration. | |
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MedLine Citation:
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PMID: 20217352 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vivo, diffusional O(2) gradients from the capillary blood to the intracellular space determine O(2) availability at the O(2) sensing molecules in the cell. With a novel technique for imaging intracellular O(2) levels using green fluorescent protein (GFP), we examined the possibility that diffusional O(2) concentration gradients might be involved in the cellular hypoxic sensing in cultured Hep3B cells. In the present study, we failed to demonstrate significant gradients of intracellular O(2) when mitochondrial respiration was maximally elevated by an uncoupler of oxidative phosphorylation. Thus, we conclude that intracellular O(2) gradients may be negligible at normal mitochondrial O(2) demand in these cells. |
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Authors:
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Eiji Takahashi; Michihiko Sato |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Advances in experimental medicine and biology Volume: 669 ISSN: 0065-2598 ISO Abbreviation: Adv. Exp. Med. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-03-10 Completed Date: 2010-05-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0121103 Medline TA: Adv Exp Med Biol Country: United States |
Other Details:
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Languages: eng Pagination: 213-7 Citation Subset: IM |
Affiliation:
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Department of Physiology, Yamagata University School of Medicine, Yamagata, Japan. eiji@med.id.yamagata-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anoxia
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metabolism* Cell Line, Tumor Cell Respiration Diffusion Humans Intracellular Space / metabolism* Mitochondria / metabolism* Oxygen / metabolism* Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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