Document Detail


Intracellular detection of laminin alpha 2 chain in skin by electron microscopy immunocytochemistry: comparison between normal and laminin alpha 2 chain deficient subjects.
MedLine Citation:
PMID:  9131649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study is to localize the alpha 2 laminin chain in normal human skin. The methods used were immuno-gold cytochemistry on cryo-ultramicrotomy sections and thin-section-fracture-label, together with electron microscopy observation. Results were compared with light microscopy peroxidase immuno-staining. Both normal skin samples and skin biopsies from laminin alpha 2 chain deficient congenital muscular dystrophy affected patients were studied. The results show that, in normal skin, the laminin alpha 2 chain is spread throughout the cytoplasm of basal keratinocytes, while it appears associated with desmosomal tonofilaments in the spinous and granular epidermal layers; in skin samples from dystrophic patients the laminin alpha 2 chain was not detectable. These data suggest that the function of the laminin alpha 2 chain is different in the epidermis as compared to that in muscle and peripheral nerve, where it is localized in the basement membrane.
Authors:
S Squarzoni; M Villanova; P Sabatelli; A Malandrini; P Toti; A Pini; L Merlini; G C Guazzi; N M Maraldi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuromuscular disorders : NMD     Volume:  7     ISSN:  0960-8966     ISO Abbreviation:  Neuromuscul. Disord.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-07-18     Completed Date:  1997-07-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9111470     Medline TA:  Neuromuscul Disord     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  91-8     Citation Subset:  IM    
Affiliation:
Istituto di Citomorfologia N.P. CNR, Bologna, Italy. squarzoni@area.bo.cnt.it
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MeSH Terms
Descriptor/Qualifier:
Humans
Immunohistochemistry
Laminin / metabolism*
Male
Microscopy, Electron
Skin / metabolism*,  ultrastructure*
Chemical
Reg. No./Substance:
0/Laminin; 0/laminin alpha 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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