Document Detail


Intracellular degradation of poly(3-hydroxybutyrate) granules of Zoogloea ramigera I-16-M.
MedLine Citation:
PMID:  1476778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intracellular degradation of poly(3-hydroxybutyrate) (PHB) in bacteria is not yet clear. The properties of the autodigestion of native PHB granules from Zoogloea ramigera I-16-M were examined. The release of D(-)-3-hydroxybutyrate was observed only at pH values higher than about 8.5 and at relatively high ionic strength (optimal concentration 200 mM NaCl). Triton X-100 and diisopropylfluorophosphate inhibited this reaction. Addition of the supernatant fraction of Z. ramigera did not increase the release of D(-)-3-hydroxybutyrate from the native PHB granules. On the other hand, using the protease-treated PHB granules from Alcaligenes eutrophus as a substrate, PHB depolymerase activity was detected in the supernatant fraction of Z. ramigera cells. The soluble PHB depolymerase showed similar properties to the enzyme in the PHB granules. Since PHB depolymerase activity was found in fractions containing D(-)-3-hydroxybutyrate oligomer hydrolase activity, which were separated by DEAE-Toyopearl or by Sephacryl S-100, it is possible that the intracellular PHB depolymerase is identical to the oligomer hydrolase which has been purified already.
Authors:
T Saito; H Saegusa; Y Miyata; T Fukui
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  FEMS microbiology reviews     Volume:  9     ISSN:  0168-6445     ISO Abbreviation:  FEMS Microbiol. Rev.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-02-11     Completed Date:  1993-02-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8902526     Medline TA:  FEMS Microbiol Rev     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  333-8     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Faculty of Science, Kanagawa University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Biodegradation, Environmental
Carboxylic Ester Hydrolases / metabolism
Hydrogen-Ion Concentration
Hydroxybutyrates / metabolism*
Osmolar Concentration
Polyesters / metabolism*
Solubility
Zoogloea / metabolism*
Chemical
Reg. No./Substance:
0/Hydroxybutyrates; 0/Polyesters; 26063-00-3/poly-beta-hydroxybutyrate; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.-/poly-beta-hydroxybutyrate depolymerase

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