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Intracellular degradation of Fusobacterium nucleatum in human gingival epithelial cells.
MedLine Citation:
PMID:  21057979     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The role of Fusobacterium nucleatum in oral health and disease is controversial. We have previously shown that F. nucleatum invades gingival epithelial cells. However, the destiny of the internalized F. nucleatum is not clear. In the present study, the intracellular destiny of F. nucleatum and its cytopathic effect on gingival epithelial cells were studied. The ability of F. nucleatum and seven other oral bacterial species to invade immortalized human gingival epithelial (HOK-16B) cells were compared by confocal microscopy and flow cytometry. F. nucleatum had the highest invasive capacity, comparable to that of Porphyromonas gingivalis, a periodontal pathogen. Confocal microscopic examination revealed colocalization of internalized F. nucleatum with endosomes and lysosomes. Examination by transmission electron microscopy revealed that most intracellular F. nucleatum was located within vesicular structures with single enclosed membranes. Furthermore, F. nucleatum could not survive within gingival epithelial cells and had no cytopathic effects on host cells. Interestingly, endosomal maturation played a role in induction of the antimicrobial peptides human beta defensin (HBD)-2 and -3 by F. nucleatum from gingival epithelial cells. F. nucleatum is destined to enter an endocytic degradation pathway after invasion and has no cytopathic effect on gingival epithelial cells, which may cast new light on the role of F. nucleatum in the pathogenesis of periodontitis.
Authors:
Suk Ji; Ji Eun Shin; Yong Cheol Kim; Youngnim Choi
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Publication Detail:
Type:  Journal Article     Date:  2010-11-05
Journal Detail:
Title:  Molecules and cells     Volume:  30     ISSN:  0219-1032     ISO Abbreviation:  Mol. Cells     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-02-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  519-26     Citation Subset:  IM    
Affiliation:
Department of Oromaxillofacial Infection and Immunity, Brain Korea 21 CLS, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, 110-749, Korea.
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