Document Detail


Intracellular cytokine staining for TGF-beta.
MedLine Citation:
PMID:  11684136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
TGF-beta is a well-described immunoregulatory molecule that is produced by most cell types. Many studies have been aimed at investigating the role played by TGF-beta in different cell types and situations. Most methods of measuring TGF-beta have previously relied on enzyme-linked immunosorbent assay (ELISA) or assays of its anti-proliferative effects on various cell lines. Both assays, though useful, cannot be used to effectively identify the cells that are producing TGF-beta in a mixture of cells. It is especially important to know the source and dynamics of TGF-beta secretion in cell culture studies since most cell types are known to be capable of producing TGF-beta. We describe here a technique of qualitative and quantitative measurement of TGF-beta production using flow cytometry. Previous work by others has led to the production of polyclonal and monoclonal antibodies to human and other mammalian TGF-beta. We have developed an intracellular cytokine staining for human TGF-beta using a monoclonal antibody, TB21.
Authors:
M L Garba; J A Frelinger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  258     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-10-30     Completed Date:  2001-12-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  193-8     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, University of North Carolina, CB#7290 MEJ, Chapel Hill, NC 27599-7290, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Cell Line
Flow Cytometry / methods
Humans
Leukocytes, Mononuclear / immunology
Staining and Labeling / methods*
Transforming Growth Factor beta / analysis*,  biosynthesis,  immunology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Transforming Growth Factor beta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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