Document Detail

Intracellular concentrations of anidulafungin in different compartments of the peripheral blood.
MedLine Citation:
PMID:  23317969     Owner:  NLM     Status:  Publisher    
Pharmacokinetic studies usually focus on serum concentrations, hence little is known about intracellular drug concentrations. In this study, we determined the concentration of anidulafungin within different cellular compartments of the peripheral blood. Blood samples (n=17) from patients receiving anidulafungin were collected and separated by double-discontinuous Ficoll-Hypaque density gradient centrifugation. Intracellular concentrations within the obtained cells, i.e. peripheral blood mononuclear cells (PBMCs), polymorphonuclear leucocytes (PMNs) and red blood cells (RBCs), were determined by liquid chromatography tandem mass spectrometry (LC-MS/MS). Within the PBMCs and PMNs, the intracellular concentration of anidulafungin was significantly increased compared with the plasma concentration (P<0.001). However, the concentration within RBCs did not significantly differ from the plasma concentration. Anidulafungin reaches high concentrations in human PBMCs and PMNs and is present in RBCs. In vitro data showed that intracellular uptake of anidulafungin by PBMCs depends on the albumin concentration of the surrounding medium, but only at low, i.e. non-physiological, protein concentrations.
Fedja Farowski; Oliver A Cornely; Jörg J Vehreschild; Martin Wiesen; Angela Steinbach; Maria J G T Vehreschild; Carsten Müller
Related Documents :
23235269 - Biochemical effects of phosphate-containing replacement fluid for continuous venovenous...
8403119 - Plasma cholinesterase deficiency in a neonate.
24834129 - Effect of systemic inflammation on level of ferritin seminal in chronic renal male pati...
24644009 - Vitamin d levels in jamaican patients with systemic lupus erythematosus.
23566409 - Increased immunoglobulin g4-positive plasma cells in collagenous sprue.
23164229 - Effect of photoperiod and heat stress in the third trimester of gestation on milk produ...
2338219 - Extraocular control of seasonal reproduction in female tree sparrows (spizella arborea).
24430779 - Iron availability is increased in individual human ovarian follicles in close proximity...
16737209 - Altered levels of acute phase proteins in the plasma of patients with schizophrenia.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-11
Journal Detail:
Title:  International journal of antimicrobial agents     Volume:  -     ISSN:  1872-7913     ISO Abbreviation:  Int. J. Antimicrob. Agents     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9111860     Medline TA:  Int J Antimicrob Agents     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Department of Internal Medicine, University of Cologne, Cologne, Germany. Electronic address:
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Prevalence and risk factors for self-reported asthma in an adult Indian population: a cross-sectiona...
Next Document:  Imaging and management of atherosclerotic renal artery stenosis.