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Intracellular concentrations of anidulafungin in different compartments of the peripheral blood.
MedLine Citation:
PMID:  23317969     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Pharmacokinetic studies usually focus on serum concentrations, hence little is known about intracellular drug concentrations. In this study, we determined the concentration of anidulafungin within different cellular compartments of the peripheral blood. Blood samples (n=17) from patients receiving anidulafungin were collected and separated by double-discontinuous Ficoll-Hypaque density gradient centrifugation. Intracellular concentrations within the obtained cells, i.e. peripheral blood mononuclear cells (PBMCs), polymorphonuclear leucocytes (PMNs) and red blood cells (RBCs), were determined by liquid chromatography tandem mass spectrometry (LC-MS/MS). Within the PBMCs and PMNs, the intracellular concentration of anidulafungin was significantly increased compared with the plasma concentration (P<0.001). However, the concentration within RBCs did not significantly differ from the plasma concentration. Anidulafungin reaches high concentrations in human PBMCs and PMNs and is present in RBCs. In vitro data showed that intracellular uptake of anidulafungin by PBMCs depends on the albumin concentration of the surrounding medium, but only at low, i.e. non-physiological, protein concentrations.
Authors:
Fedja Farowski; Oliver A Cornely; Jörg J Vehreschild; Martin Wiesen; Angela Steinbach; Maria J G T Vehreschild; Carsten Müller
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-11
Journal Detail:
Title:  International journal of antimicrobial agents     Volume:  -     ISSN:  1872-7913     ISO Abbreviation:  Int. J. Antimicrob. Agents     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9111860     Medline TA:  Int J Antimicrob Agents     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Affiliation:
Department of Internal Medicine, University of Cologne, Cologne, Germany. Electronic address: Fedja.Farowski@ctuc.de.
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