Document Detail


Intracellular calcium release is required for caspase-3 and -9 activation.
MedLine Citation:
PMID:  14695652     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Increase in intracellular Ca2+ [Ca2+]i regulates many biological functions including apoptosis, but the protein(s) linking [Ca2+]i and apoptosis are not completely understood. We have previously shown that IP3R-deficient cells are resistant to T-cell receptor (TCR)-induced apoptosis due to lack of Ca2+ release from endoplasmic reticulum (ER) and calcineurin activation. Here we show that caspase-9 and -3 are not activated in IP3R-deficient cells after TCR stimulation, consistent with the resistance of these cells to apoptosis. However, we also demonstrate that Bcl-2 expression in IP3R-deficient cells is comparable to control cells. Taken together, these results strongly suggest that IP3R-mediated Ca2+ release plays a critical role in regulating the activity of caspases-3 and -9 independent of Bcl-2.
Authors:
Lee Tantral; Krishnamurthy Malathi; Shinya Kohyama; Michael Silane; Alejandro Berenstein; Thottala Jayaraman
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  22     ISSN:  0263-6484     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:    2004 Jan-Feb
Date Detail:
Created Date:  2003-12-25     Completed Date:  2004-08-17     Revised Date:  2012-04-18    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  35-40     Citation Subset:  IM    
Copyright Information:
Copyright 2003 John Wiley & Sons, Ltd.
Affiliation:
Vascular Biology Laboratory, Department of Medicine, St. Luke's Roosevelt Hospital Center, New York, NY 10025, USA.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
Blotting, Western
Calcium / metabolism*
Calcium Channels / chemistry
Calcium Signaling*
Caspase 3
Caspase 9
Caspases / chemistry,  metabolism*
Enzyme Activation
Humans
Inositol 1,4,5-Trisphosphate Receptors
Jurkat Cells
Proto-Oncogene Proteins c-bcl-2 / chemistry
Receptors, Antigen, T-Cell / chemistry
Receptors, Cytoplasmic and Nuclear / chemistry
Grant Support
ID/Acronym/Agency:
R15 AI089518-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Calcium Channels; 0/ITPR1 protein, human; 0/Inositol 1,4,5-Trisphosphate Receptors; 0/Proto-Oncogene Proteins c-bcl-2; 0/Receptors, Antigen, T-Cell; 0/Receptors, Cytoplasmic and Nuclear; 7440-70-2/Calcium; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/CASP9 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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