| Intracellular bevacizumab reduces phagocytotic uptake in RPE cells. | |
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MedLine Citation:
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PMID: 20169356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: We have previously shown that bevacizumab, but not ranibizumab, is taken up by porcine RPE cells. In this study, the effects of bevacizumab and ranibizumab on proliferation, wound healing and phagocytosis of the RPE were investigated. METHODS: Primary porcine RPE cell culture were prepared from fresh eyes, cultivated and treated with clinically relevant concentrations of bevacizumab or ranibizumab respectively. Proliferation was investigated in a proliferation assay, wound healing in a wound scratch assay and phagocytosis was investigated by feeding RPE cells photoreceptor outer segment-opsonized FITC-labeled latex beads. RESULTS: Bevacizumab, and to a lesser extend ranibizumab, impair the proliferation of RPE cells but do not affect wound healing. Bevacizumab, but not ranibizumab, reduces the phagocytotic function of RPE cells. CONCLUSIONS: The uptake of bevacizumab reduces phagocytosis in RPE cells, which indicates possible long-term effects of repeated bevacizumab treatment. |
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Authors:
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Alexa Klettner; Friederike Möhle; Johann Roider |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-19 |
Journal Detail:
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Title: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie Volume: 248 ISSN: 1435-702X ISO Abbreviation: Graefes Arch. Clin. Exp. Ophthalmol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-04-26 Completed Date: 2010-07-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8205248 Medline TA: Graefes Arch Clin Exp Ophthalmol Country: Germany |
Other Details:
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Languages: eng Pagination: 819-24 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, University of Kiel,, Arnold-Heller-Str. 3, Haus 25, 24105, Kiel, Germany. aklettner@auge.uni-kiel.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Angiogenesis Inhibitors
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pharmacology* Animals Antibodies, Monoclonal / pharmacology* Cell Proliferation / drug effects Cells, Cultured Phagocytosis / drug effects* Retinal Photoreceptor Cell Outer Segment / metabolism Retinal Pigment Epithelium / cytology, physiology* Swine Wound Healing / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Angiogenesis Inhibitors; 0/Antibodies, Monoclonal; 0/bevacizumab; 0/ranibizumab |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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