| Intracellular ascorbic acid enhances the DNA single-strand breakage and toxicity induced by peroxynitrite in U937 cells. | |
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MedLine Citation:
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PMID: 11368779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A well-established protocol to increase the intracellular content of ascorbic acid was used to investigate the effects of the vitamin on DNA single-strand breakage and toxicity mediated by authentic peroxynitrite (ONOO(-)) in U937 cells. This protocol involved exposure for 60 min to 100 microM dehydroascorbic acid, which was taken up by the cells and converted into ascorbic acid via a GSH-independent mechanism. At the time of exposure to ONOO(-), which was performed in fresh saline immediately after loading with dehydroascorbic acid, the vitamin present in the cells was all in its reduced form. It was found that, in cells that are otherwise ascorbate-deficient, an increase in their ascorbic acid content does not prevent, but rather enhances, the DNA-damaging and lethal responses mediated by exogenous ONOO(-). These results therefore suggest that acute supplementation of ascorbic acid can be detrimental for individuals with pathologies associated with a decrease in ascorbic acid and in which ONOO(-) is known to promote deleterious effects. |
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Authors:
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A Guidarelli; R De Sanctis; B Cellini; M Fiorani; M Dachà; O Cantoni |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 356 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-05-22 Completed Date: 2001-07-19 Revised Date: 2012-02-22 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: England |
Other Details:
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Languages: eng Pagination: 509-13 Citation Subset: IM |
Affiliation:
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Istituto di Farmacologia e Farmacognosia, Via S. Chiara 27, 61029 Urbino, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ascorbic Acid
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metabolism*,
toxicity DNA Damage* Dehydroascorbic Acid / metabolism, pharmacology Ferricyanides / metabolism Free Radical Scavengers / pharmacology Humans Iron Chelating Agents / pharmacology Nitrates / toxicity* Oxidation-Reduction U937 Cells |
| Grant Support | |
ID/Acronym/Agency:
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1110//Telethon |
| Chemical | |
Reg. No./Substance:
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0/Ferricyanides; 0/Free Radical Scavengers; 0/Iron Chelating Agents; 0/Nitrates; 13408-62-3/hexacyanoferrate III; 26404-66-0/peroxynitric acid; 490-83-5/Dehydroascorbic Acid; 50-81-7/Ascorbic Acid |
| Comments/Corrections | |
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