Document Detail


Intracellular acid phosphatase content and ability of different macrophage populations to kill Nocardia asteroides.
MedLine Citation:
PMID:  3881345     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been reported that the activity of lysosomal acid phosphatase decreases inversely with numbers of ingested virulent Nocardia spp. in normal murine peritoneal and alveolar macrophages. These studies suggested that this relationship correlated with the effectiveness of these macrophage populations in killing Nocardia asteroides. Experiments were designed to determine if acid phosphatase activity is affected by infection with N. asteroides in four different macrophage populations isolated from normal and nocardia-immunized mice. Macrophages were also tested simultaneously for their ability to kill N. asteroides. Peritoneal, alveolar, and splenic macrophages and Kupffer cells were infected in vitro with strains of N. asteroides of differing virulence. Uptake and killing assays were performed. Acid phosphatase levels and numbers of intracellular nocardiae were quantitated in the same macrophages, using a computer-assisted cytophotometry system. Acid phosphatase activity decreased inversely with numbers of intracellular nocardiae in macrophages that could not kill or inhibit this pathogen. Acid phosphatase activity was not significantly changed in macrophages that inhibited growth of, but did not kill, N. asteroides, whereas activity was increased or enhanced in macrophages that killed most of the ingested nocardiae. The order of nocardicidal effectiveness (and resistance to enzyme activity reduction with infection) for normal macrophages was splenic greater than peritoneal greater than alveolar greater than Kupffer. In contrast, the order of these two parameters for macrophages isolated from immunized mice was Kupffer greater than peritoneal greater than alveolar greater than splenic. These results demonstrate that lysosomal acid phosphatase activity is an effective marker of the ability of macrophages to inhibit growth of and kill N. asteroides and that macrophages isolated from different anatomical sites differ functionally from each other with respect to nocardicidal and acid phosphatase activities.
Authors:
C M Black; B L Beaman; R M Donovan; E Goldstein
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  47     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-03-21     Completed Date:  1985-03-21     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  375-83     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / analysis*
Animals
Female
Humans
Kupffer Cells / physiology
Lysosomes / enzymology
Macrophages / enzymology*,  physiology
Mice
Mice, Inbred BALB C
Nocardia asteroides*
Peritoneal Cavity / cytology
Phagocytosis*
Pulmonary Alveoli / cytology
Spleen / cytology
Grant Support
ID/Acronym/Agency:
AI-19579/AI/NIAID NIH HHS; ES-00628/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
EC 3.1.3.2/Acid Phosphatase
Comments/Corrections

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