Document Detail


Intracellular accumulation of the anti-CD20 antibody 1F5 in B-lymphoma cells.
MedLine Citation:
PMID:  12171904     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In previous experiments, 125I-labeled 1F5 (anti-CD20)was found to kill B-lymphoma cells efficiently and specifically.Unexpectedly, the number of antibody (Ab) molecules taken up per cell was much larger than the number of antigen sites on the cell surface. The present studies were designed to explain this apparent discrepancy. Incubation with fluorophore-conjugated 1F5, using the Raji cell line, demonstrated that the Ab accumulated in large amounts in a juxtanuclear spot. Double labeling showed that the same spot was labeled by transferrin, but the transferrin labeling was much faster (45 min versus 18 h). Experiments with brefeldin A demonstrated that the spot stained was distinct from the Golgi cisternae; thus, it appears to be the endocytic recycling compartment. A fluorescent Fab fragment of 1F5 produced much weaker, barely detectable staining of the juxtanuclear spot. Experiments with three other B-lymphoma cell lines demonstrated marked heterogeneity among them. With Ramos cells, 1F5 and transferrin localized to multiple smaller intracellular spots, rather than a single large spot. There were also major differences between different Abs to CD20, as tested on Raji cells. Rituximab showed some staining of the juxtanuclear spot, but not as homogeneously as the staining with 1F5. B1 and L27 were not tested as thoroughly but did not appear to stain the juxtanuclear spot. Such internalization may have a major impact on the therapy of this tumor type with conjugates of anti-CD20 Abs. However, internalization did not correlate with sensitivity to specific killing by 125I-labeled 1F5.
Authors:
Rosana B Michel; M Jules Mattes
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical cancer research : an official journal of the American Association for Cancer Research     Volume:  8     ISSN:  1078-0432     ISO Abbreviation:  Clin. Cancer Res.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-12     Completed Date:  2003-02-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9502500     Medline TA:  Clin Cancer Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2701-13     Citation Subset:  IM    
Affiliation:
Center for Molecular Medicine and Immunology, Belleville, New Jersey 07109, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies / chemistry*
Antibody Specificity
Antigens, CD20 / biosynthesis,  immunology*
Binding, Competitive
Brefeldin A / pharmacology
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Epitopes
Golgi Apparatus / metabolism
Humans
Lymphoma, B-Cell / immunology*,  metabolism
Microscopy, Fluorescence
Protein Binding
Protein Synthesis Inhibitors / pharmacology
Time Factors
Transferrin / metabolism
Tumor Cells, Cultured
Ultraviolet Rays
Up-Regulation
Grant Support
ID/Acronym/Agency:
CA87059/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD20; 0/Epitopes; 0/Protein Synthesis Inhibitors; 11096-37-0/Transferrin; 20350-15-6/Brefeldin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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