Document Detail


The Intracellular Metabolism of Legionella by Isotopologue Profiling.
MedLine Citation:
PMID:  23150394     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Metabolic pathways and fluxes can be analyzed under in vivo conditions by incorporation experiments using general (13)C-labeled precursors. On the basis of the isotopologue compositions in amino acids or other metabolites, the incorporation rates of the supplied precursors and the pathways of their utilization can be studied in considerable detail. In this chapter, the method of isotopologue profiling is illustrated with recent work on the metabolism of intracellular living Legionella pneumophila.
Authors:
Klaus Heuner; Wolfgang Eisenreich
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  954     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  163-181     Citation Subset:  -    
Affiliation:
Working group "Cellular Interactions of Bacterial Pathogens", Robert Koch-Institut, Berlin, Germany, heunerk@rki.de.
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