| Intracellular delivery of bioactive molecules using light-addressable nanocapsules. | |
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MedLine Citation:
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PMID: 21117640 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper describes a method by which molecules that are impermeable to cells are encapsulated in dye-sensitized lipid nanocapsules for delivery into cells via endocytosis. Once inside the cells, the molecules are released from the lipid nanocapsules into the cytoplasm with a single nanosecond pulse from a laser in the far red (645 nm). We demonstrate this method with the intracellular release of the second messenger IP(3) in CHO-M1 cells and report that calcium responses from the cells changed from a sustained increase to a transient spike when the average number of IP(3) released is decreased below 50 molecules per nanocapsule. We also demonstrate the delivery of a 23 kDa O(6)-alkylguanine-DNA alkyltransferase (AGT) fusion protein into Ba/F3 cells to inhibit a key player BCR-ABL in the apoptotic pathway. We show that an average of ∼8 molecules of the inhibitor is sufficient to induce apoptosis in the majority of Ba/F3 cells. |
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Authors:
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Kimberly A D Gregersen; Zachary B Hill; Jennifer C Gadd; Bryant S Fujimoto; Dustin J Maly; Daniel T Chiu |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-11-30 |
Journal Detail:
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Title: ACS nano Volume: 4 ISSN: 1936-086X ISO Abbreviation: ACS Nano Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-28 Completed Date: 2011-03-23 Revised Date: 2012-10-09 |
Medline Journal Info:
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Nlm Unique ID: 101313589 Medline TA: ACS Nano Country: United States |
Other Details:
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Languages: eng Pagination: 7603-11 Citation Subset: IM |
Affiliation:
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Chemistry Department, University of Washington, Seattle, Washington, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport CHO Cells Calcium / metabolism Cricetinae Cricetulus Enzyme Inhibitors / chemistry, pharmacology Fusion Proteins, bcr-abl / antagonists & inhibitors, metabolism HEK293 Cells Humans Inositol 1,4,5-Trisphosphate / chemistry, metabolism Inositol 1,4,5-Trisphosphate Receptors / metabolism Intracellular Space / metabolism*, radiation effects* Lasers Light* Lipids / chemistry Nanocapsules* / chemistry O(6)-Methylguanine-DNA Methyltransferase / antagonists & inhibitors, metabolism Photolysis Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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1T32CA138312-01/CA/NCI NIH HHS; AG 029574/AG/NIA NIH HHS; R21 AG029574-01A1/AG/NIA NIH HHS; R21 AG029574-02/AG/NIA NIH HHS; R21 AG029574-03/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Fusion Proteins, bcr-abl; 0/Inositol 1,4,5-Trisphosphate Receptors; 0/Lipids; 0/Nanocapsules; 7440-70-2/Calcium; 85166-31-0/Inositol 1,4,5-Trisphosphate; EC 2.1.1.63/O(6)-Methylguanine-DNA Methyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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