| Intracellular calcium oscillations in articular chondrocytes induced by basic calcium phosphate crystals lead to cartilage degradation. | |
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MedLine Citation:
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PMID: 22885567 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Basic calcium phosphate (BCP) crystals, including octacalcium phosphate (OCP), carbonated-apatite (CA) and hydroxyapatite (HA) crystals are associated with destructive forms of osteoarthritis. Mechanisms of BCP-induced cartilage breakdown remain incompletely understood. We assessed the ability of BCP to induce changes in intracellular calcium (iCa(2+)) content and oscillations and the role of iCa(2+) in BCP-induced cartilage degradation. METHODS: Bovine articular chondrocytes (BACs) and bovine cartilage explants (BCEs) were stimulated with BCP or monosodium urate (MSU) crystals. iCa(2+) levels were determined by spectrofluorimetry and oscillations by confocal microscopy. mRNA expression of matrix metalloproteinase 3 (MMP-3), a disintegrin and metalloprotease with thrombospondin-like motifs 4 (ADAMTS-4) and ADAMTS-5 was assessed by quantitative real-time PCR. Glycosaminoglycan (GAG) release was measured in the supernatants of BCE cultures. RESULTS: All three BCP crystals significantly increased iCa(2+) content. OCP also induced iCa(2+) oscillations. Rate of BACs displaying iCa(2+) oscillations increased over time, with a peak after 20 min of stimulation. OCP-induced iCa(2+) oscillations involved both extracellular Ca(2+) (eCa(2+)) influx and iCa(2+) stores. Indeed, OCP-induced iCa(2+) oscillations decreased rapidly in Ca(2+)-free medium. Both voltage- and non-voltage-dependent Ca(2+) channels were involved in eCa(2+) influx. BCP crystal-induced variation in iCa(2+) content was associated with BCP crystal-induced cartilage matrix degradation. However, iCa²(+) was not associated with OCP crystal-induced mRNA expression of MMP-3, ADAMTS-4 or ADAMTS-5. CONCLUSION: BCP crystals can induce variation in iCa(2+) content and oscillations in articular chondrocytes. Furthermore, BCP crystal-induced changes in iCa(2+) content play a pivotal role in BCP catabolic effects on articular cartilage. |
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Authors:
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C Nguyen; M Lieberherr; C Bordat; F Velard; D Côme; F Lioté; H-K Ea |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-08-08 |
Journal Detail:
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Title: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society Volume: 20 ISSN: 1522-9653 ISO Abbreviation: Osteoarthr. Cartil. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-09-21 Completed Date: 2013-03-07 Revised Date: 2013-04-05 |
Medline Journal Info:
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Nlm Unique ID: 9305697 Medline TA: Osteoarthritis Cartilage Country: England |
Other Details:
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Languages: eng Pagination: 1399-408 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Univ Paris Diderot, Sorbonne Paris Cité, INSERM, UMR-S 606, F-75205 Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium Channel Blockers / pharmacology Calcium Phosphates / metabolism, pharmacology* Calcium Signaling / drug effects*, physiology Cartilage, Articular / drug effects, metabolism, pathology* Cattle Cells, Cultured Chondrocytes / drug effects, metabolism, pathology* Crystallization Drug Antagonism Female Proteoglycans / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Calcium Channel Blockers; 0/Calcium Phosphates; 0/Proteoglycans; 97Z1WI3NDX/calcium phosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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