| Intracellular Ca2+ responses and cell volume regulation upon cholinergic and purinergic stimulation in an immortalized salivary cell line. | |
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MedLine Citation:
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PMID: 20572856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The water channel aquaporin 5 (AQP5) seems to play a key role in salivary fluid secretion and appears to be critical in the cell volume regulation of acinar cells. Recently, the cation channel transient potential vanilloid receptor 4 (TRPV4) was shown to be functionally connected to AQP5 and also to cell volume regulation in salivary glands. We used the Simian virus 40 (SV40) immortalized cell line SMG C10 from the rat submandibular salivary gland to investigate the effect of ATP and the neurotransmitter analogue carbachol on Ca(2+) signalling and cell volume regulation, as well as the involvement of TRPV4 in the responses. We used fura-2-AM imaging, cell volume measurements, and western blotting. Both carbachol and ATP increased the concentration of intracellular Ca(2+), but no volume changes could be measured. Inhibition of TRPV4 with ruthenium red impaired both ATP- and carbachol-stimulated Ca(2+) signals. Peak Ca(2+) signalling during hyposmotic exposure was significantly decreased following inhibition of TRPV4, while the cells' ability to volume regulate appeared to be unaffected. These results show that in the SMG C10 cells, simulation of nervous stimulation did not induce cell swelling, although the cells had intact volume regulatory mechanisms. Furthermore, even though Ca(2+) signals were not needed for this volume regulation, TRPV4 seems to play a role during ATP and carbachol stimulation. |
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Authors:
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Marit H Aure; Asbjørn Røed; Hilde Kanli Galtung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of oral sciences Volume: 118 ISSN: 1600-0722 ISO Abbreviation: Eur. J. Oral Sci. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-24 Completed Date: 2010-09-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9504563 Medline TA: Eur J Oral Sci Country: Denmark |
Other Details:
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Languages: eng Pagination: 237-44 Citation Subset: D; IM |
Affiliation:
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Department of Oral Biology, Faculty of Dentistry, University of Oslo, Blindern, Oslo, Norway. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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pharmacology Animals Aquaporin 5 / drug effects Atropine / pharmacology Calcium Signaling / drug effects* Carbachol / pharmacology Cell Culture Techniques Cell Line, Transformed Cell Size / drug effects Cholinergic Agents / pharmacology* Cholinergic Agonists / pharmacology Fluorescent Dyes / diagnostic use Fura-2 / analogs & derivatives, diagnostic use Muscarinic Antagonists / pharmacology Osmotic Pressure / physiology Purines / pharmacology* Pyridoxal Phosphate / analogs & derivatives, pharmacology Rats Receptors, Purinergic P2 / antagonists & inhibitors Ruthenium Red / pharmacology Submandibular Gland / cytology, drug effects* Suramin / pharmacology TRPV Cation Channels / antagonists & inhibitors, drug effects |
| Chemical | |
Reg. No./Substance:
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0/Aqp5 protein, rat; 0/Aquaporin 5; 0/Cholinergic Agents; 0/Cholinergic Agonists; 0/Fluorescent Dyes; 0/Muscarinic Antagonists; 0/Purines; 0/Receptors, Purinergic P2; 0/TRPV Cation Channels; 0/Trpv4 protein, rat; 0/purinergic P2X receptor; 105344-37-4/fura-2-am; 11103-72-3/Ruthenium Red; 145-63-1/Suramin; 149017-66-3/pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid; 51-55-8/Atropine; 51-83-2/Carbachol; 54-47-7/Pyridoxal Phosphate; 56-65-5/Adenosine Triphosphate; 96314-98-6/Fura-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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