Document Detail


Intracellular Ca2+ responses and cell volume regulation upon cholinergic and purinergic stimulation in an immortalized salivary cell line.
MedLine Citation:
PMID:  20572856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The water channel aquaporin 5 (AQP5) seems to play a key role in salivary fluid secretion and appears to be critical in the cell volume regulation of acinar cells. Recently, the cation channel transient potential vanilloid receptor 4 (TRPV4) was shown to be functionally connected to AQP5 and also to cell volume regulation in salivary glands. We used the Simian virus 40 (SV40) immortalized cell line SMG C10 from the rat submandibular salivary gland to investigate the effect of ATP and the neurotransmitter analogue carbachol on Ca(2+) signalling and cell volume regulation, as well as the involvement of TRPV4 in the responses. We used fura-2-AM imaging, cell volume measurements, and western blotting. Both carbachol and ATP increased the concentration of intracellular Ca(2+), but no volume changes could be measured. Inhibition of TRPV4 with ruthenium red impaired both ATP- and carbachol-stimulated Ca(2+) signals. Peak Ca(2+) signalling during hyposmotic exposure was significantly decreased following inhibition of TRPV4, while the cells' ability to volume regulate appeared to be unaffected. These results show that in the SMG C10 cells, simulation of nervous stimulation did not induce cell swelling, although the cells had intact volume regulatory mechanisms. Furthermore, even though Ca(2+) signals were not needed for this volume regulation, TRPV4 seems to play a role during ATP and carbachol stimulation.
Authors:
Marit H Aure; Asbjørn Røed; Hilde Kanli Galtung
Related Documents :
19846756 - Deregulation of apoptotic volume decrease and ionic movements in multidrug-resistant tu...
5891056 - Electronic separation of biological cells by volume.
15614486 - The v-atpase inhibitors concanamycin a and bafilomycin a lead to golgi swelling in toba...
7082266 - Relationships between dna content and cell morphometric parameters in reptiles.
24752676 - Synthesis and characterization of novel zinc phthalocyanines as potential photosensitiz...
1362196 - Retinoic acid increases cd15 expression in immortalized rat astrocytes.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of oral sciences     Volume:  118     ISSN:  1600-0722     ISO Abbreviation:  Eur. J. Oral Sci.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-24     Completed Date:  2010-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9504563     Medline TA:  Eur J Oral Sci     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  237-44     Citation Subset:  D; IM    
Affiliation:
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Blindern, Oslo, Norway.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / pharmacology
Animals
Aquaporin 5 / drug effects
Atropine / pharmacology
Calcium Signaling / drug effects*
Carbachol / pharmacology
Cell Culture Techniques
Cell Line, Transformed
Cell Size / drug effects
Cholinergic Agents / pharmacology*
Cholinergic Agonists / pharmacology
Fluorescent Dyes / diagnostic use
Fura-2 / analogs & derivatives,  diagnostic use
Muscarinic Antagonists / pharmacology
Osmotic Pressure / physiology
Purines / pharmacology*
Pyridoxal Phosphate / analogs & derivatives,  pharmacology
Rats
Receptors, Purinergic P2 / antagonists & inhibitors
Ruthenium Red / pharmacology
Submandibular Gland / cytology,  drug effects*
Suramin / pharmacology
TRPV Cation Channels / antagonists & inhibitors,  drug effects
Chemical
Reg. No./Substance:
0/Aqp5 protein, rat; 0/Aquaporin 5; 0/Cholinergic Agents; 0/Cholinergic Agonists; 0/Fluorescent Dyes; 0/Muscarinic Antagonists; 0/Purines; 0/Receptors, Purinergic P2; 0/TRPV Cation Channels; 0/Trpv4 protein, rat; 0/purinergic P2X receptor; 105344-37-4/fura-2-am; 11103-72-3/Ruthenium Red; 145-63-1/Suramin; 149017-66-3/pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid; 51-55-8/Atropine; 51-83-2/Carbachol; 54-47-7/Pyridoxal Phosphate; 56-65-5/Adenosine Triphosphate; 96314-98-6/Fura-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Developmental changes in cellular and extracellular structural macromolecules in the secondary palat...
Next Document:  Protein and mucin retention on oral mucosal surfaces in dry mouth patients.